Rat Platelet Derived Growth Factor D (PDGFD) ELISA Kit | PDGFD elisa kit

Rat Platelet Derived Growth Factor D (PDGFD) ELISA Kit

Cat. Num. AAA2024098

• Gene Names: PDGFD; IEGF; SCDGFB; MSTP036; SCDGF-B
• Reactivity: Rat
• Synonyms: Platelet Derived Growth Factor D (PDGFD); Rat Platelet Derived Growth Factor D (PDGFD) ELISA Kit; PDGF-D; IEGF; SCDGF-B; Spinal Cord Derived Growth Factor B; Iris-expressed growth factor; Platelet-derived growth factor D; receptor-binding form/latent form; PDGFD elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Platelet Derived Growth Factor D (PDGFD).; No significant cross-reactivity or interference between Platelet Derived Growth Factor D (PDGFD) and analogues was observed.
• Sequence Length: 364

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.125ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Derived Growth Factor D (PDGFD) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Derived Growth Factor D (PDGFD) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDGFD elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Derived Growth Factor D (PDGFD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Platelet Derived Growth Factor D (PDGFD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet Derived Growth Factor D (PDGFD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Platelet Derived Growth Factor D (PDGFD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PDGFD elisa kit

Cytokine

NCBI and Uniprot Product Information

• NCBI GI #: 15451921
• NCBI GeneID: 80310
• NCBI Accession #: NP_149126.1
• NCBI GenBank Nucleotide #: NM_033135.3
• UniProt Accession #: Q9GZP0; Q9BWV5; A8K9T6
• Molecular Weight: 42,167 Da
• NCBI Official Full Name: platelet-derived growth factor D isoform 2
• NCBI Official Synonym Full Names: platelet derived growth factor D
• NCBI Official Symbol: PDGFD
• NCBI Official Synonym Symbols: IEGF; SCDGFB; MSTP036; SCDGF-B
• NCBI Protein Information: platelet-derived growth factor D
• UniProt Protein Name: Platelet-derived growth factor D
• Protein Family: Platelet-derived growth factor
• UniProt Gene Name: PDGFD
• UniProt Synonym Gene Names: IEGF; SCDGFB; PDGF-D; SCDGF-B; PDGFD latent form; PDGFD receptor-binding form
• UniProt Entry Name: PDGFD_HUMAN

NCBI Description

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. This gene product only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. Two splice variants have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PDGFD: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Plays an important role in wound healing. Induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. Can initiate events that lead to a mesangial proliferative glomerulonephritis, including influx of monocytes and macrophages and production of extracellular matrix. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: Golgi membrane; endoplasmic reticulum lumen; extracellular region

Molecular Function: growth factor activity; platelet-derived growth factor receptor binding

Biological Process: multicellular organismal development; positive regulation of cell division; platelet-derived growth factor receptor signaling pathway; regulation of peptidyl-tyrosine phosphorylation