Rat Platelet Derived Growth Factor D ELISA Kit | PDGF-D elisa kit

Rat Platelet Derived Growth Factor D ELISA Kit

Cat. Num. AAA760220

• Gene Names: PDGFD; IEGF; SCDGFB; MSTP036; SCDGF-B
• Reactivity: Rat
• Synonyms: Platelet Derived Growth Factor D; Rat Platelet Derived Growth Factor D ELISA Kit; PDGF-D/IEGFMSTP036/Iris-expressed growth factor/PDGF-D/platelet derived growth factor D/SCDGF-BMGC26867/SCDGFBplatelet-derived growth factor D/spinal cord derived growth factor B/Spinal cord-derived growth factor B/spinal cord-derived growth factor-B; PDGF-D elisa kit

• Reactivity: Rat
• Sequence Length: 364

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for PDGF-D elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Rat PDGF-D antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat PDGF-D antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat PDGF-D amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat PDGF-D can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 15451921
• NCBI GeneID: 80310
• NCBI Accession #: NP_149126.1
• NCBI GenBank Nucleotide #: NM_033135.3
• Molecular Weight: 42,167 Da
• NCBI Official Full Name: platelet-derived growth factor D isoform 2
• NCBI Official Synonym Full Names: platelet derived growth factor D
• NCBI Official Symbol: PDGFD
• NCBI Official Synonym Symbols: IEGF; SCDGFB; MSTP036; SCDGF-B
• NCBI Protein Information: platelet-derived growth factor D
• UniProt Protein Name: Platelet-derived growth factor D
• UniProt Gene Name: PDGFD
• UniProt Synonym Gene Names: IEGF; SCDGFB; PDGF-D; SCDGF-B; PDGFD latent form; PDGFD receptor-binding form
• UniProt Entry Name: PDGFD_HUMAN

NCBI Description

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. This gene product only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. Two splice variants have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PDGFD: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Plays an important role in wound healing. Induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. Can initiate events that lead to a mesangial proliferative glomerulonephritis, including influx of monocytes and macrophages and production of extracellular matrix. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi membrane

Molecular Function: growth factor activity; platelet-derived growth factor receptor binding

Biological Process: multicellular organismal development; platelet-derived growth factor receptor signaling pathway; positive regulation of cell division; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of fibroblast proliferation; positive regulation of MAP kinase activity; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid autophosphorylation; positive regulation of smooth muscle cell proliferation; regulation of peptidyl-tyrosine phosphorylation