Human Platelet Derived Growth Factor D (PDGFD) CLIA Kit | PDGFD clia kit

Human Platelet Derived Growth Factor D (PDGFD) CLIA Kit

Cat. Num. AAA2000617

• Gene Names: PDGFD; IEGF; SCDGFB; MSTP036; SCDGF-B
• Reactivity: Human
• Synonyms: Platelet Derived Growth Factor D (PDGFD); Human Platelet Derived Growth Factor D (PDGFD) CLIA Kit; PDGF-D; IEGF; SCDGF-B; Spinal Cord Derived Growth Factor B; Iris-expressed growth factor; Platelet-derived growth factor D; receptor-binding form/latent form; PDGFD clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Platelet Derived Growth Factor D (PDGFD).; No significant cross-reactivity or interference between Platelet Derived Growth Factor D (PDGFD) and analogues was observed.
• Sequence Length: 364

• Assay Type: Double-antibody Sandwich
• Samples: Plasma
• Detection Range: 54.9-40,000pg/mL
• Sensitivity: < 25.1pg/mL
• Application: Chemiluminescent immunoassay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Derived Growth Factor D (PDGFD) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Derived Growth Factor D (PDGFD) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDGFD clia kit

The microplate provided in this kit has been pre-coated with an antibody specific to Platelet Derived Growth Factor D (PDGFD). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Platelet Derived Growth Factor D (PDGFD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Platelet Derived Growth Factor D (PDGFD) level in the sample or standard.;

Product Categories/Family for PDGFD clia kit

Cytokine

NCBI and Uniprot Product Information

• NCBI GI #: 15451921
• NCBI GeneID: 80310
• NCBI Accession #: NP_149126.1
• NCBI GenBank Nucleotide #: NM_033135.3
• UniProt Accession #: Q9GZP0; Q9BWV5; A8K9T6
• Molecular Weight: 42,167 Da
• NCBI Official Full Name: platelet-derived growth factor D isoform 2
• NCBI Official Synonym Full Names: platelet derived growth factor D
• NCBI Official Symbol: PDGFD
• NCBI Official Synonym Symbols: IEGF; SCDGFB; MSTP036; SCDGF-B
• NCBI Protein Information: platelet-derived growth factor D
• UniProt Protein Name: Platelet-derived growth factor D
• Protein Family: Platelet-derived growth factor
• UniProt Gene Name: PDGFD
• UniProt Synonym Gene Names: IEGF; SCDGFB; PDGF-D; SCDGF-B; PDGFD latent form; PDGFD receptor-binding form
• UniProt Entry Name: PDGFD_HUMAN

NCBI Description

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. This gene product only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. Two splice variants have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PDGFD: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Plays an important role in wound healing. Induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. Can initiate events that lead to a mesangial proliferative glomerulonephritis, including influx of monocytes and macrophages and production of extracellular matrix. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Oncoprotein

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: Golgi membrane; endoplasmic reticulum lumen; extracellular region

Molecular Function: growth factor activity; platelet-derived growth factor receptor binding

Biological Process: positive regulation of cell division; multicellular organismal development; platelet-derived growth factor receptor signaling pathway; regulation of peptidyl-tyrosine phosphorylation