Rat EPO ELISA Kit | EPO elisa kit

Rat EPO PicoKine ELISA Kit

Cat. Num. AAA177385

• Reactivity: Rat
• Synonyms: EPO; Rat EPO PicoKine ELISA Kit; Erythropoietin; erythropoietin; EP; EPO alpha; Epoetin; Erythropoetin; Erythropoietin precursor; MGC138142; EPO elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant Rat Cxadr
• Sequence Length: 192

• Samples: Cell Culture Supernates, Serum And Plasma (Heparin, Edta, Citrate
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EPO elisa kit

Principle of the Assay: The Picokine Rat Cxadr Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Cxadr with a 96-well strip plate that is pre-coated with antibody specific for Cxadr. The detection antibody is a biotinylated antibody specific for Cxadr. The capture antibody is A monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Rat Cxadr with immunogen: Expression system for standard: NSO, Immunogen sequence: L20-G237. The kit is analytically validated with ready to use reagents. To measure Rat Cxadr, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Rat Cxadr in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Cxadr in the sample!!Background/Introduction: CXADR(Coxsackie virus and adenovirus receptor) is a protein that in humans is encoded by the CXADR gene, also known as CAR,CVB3-binding protein, this gene is mapped to 11q11. Coxsackievirus B-adenovirus receptor. The CAR cDNA encodes a predicted 365-amino acid polypeptide that contains a single transmembrane domain and is a member of the immunoglobulin superfamily. By Northern blot analysis, they detected highest expression of 1.4-kb and 6-kb CXADR transcripts in pancreas, brain, heart, small intestine, testis, and prostate, lower expression in liver and lung, and no expression in kidney, placenta, peripheral blood leukocytes, thymus, and spleen. In comparison, mouse Cxadr showed highest expression in liver, and lower levels in kidney, heart, lung, and brain. The protein encoded by this gene is a type I membrane receptor for group B coxsackie viruses and subgroup C adenoviruses. Pseudogenes of this gene are found on chromosomes 15, 18, and 21. CAR is strongly expressed in the developing central nervous system. It functions as a homophilic and also as a heterophilic cell adhesion molecule through its interactions with extracellular matrix glycoproteins, such as: fibronectin, agrin, laminin-1 and tenascin-R.

NCBI and Uniprot Product Information

• NCBI GI #: 232064
• NCBI GeneID: 24335
• UniProt Accession #: P29676; P70504
• Molecular Weight: 21,286 Da
• NCBI Official Full Name: Erythropoietin
• NCBI Official Synonym Full Names: erythropoietin
• NCBI Official Symbol: Epo
• NCBI Protein Information: erythropoietin
• UniProt Protein Name: Erythropoietin
• Protein Family: Erythropoietin
• UniProt Gene Name: Epo
• UniProt Entry Name: EPO_RAT

NCBI Description

acts as a regulator of erythropoiesis; may facilitate oxygen delivery in response to hypoxia [RGD, Feb 2006]

Uniprot Description

Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.