Mouse TRKC ELISA Kit | TRKC elisa kit

Mouse TRKC PicoKine ELISA Kit

Cat. Num. AAA1751578

• Gene Names: NTRK3; TRKC; GP145-TrkC; gp145(trkC)
• Reactivity: Mouse
• Synonyms: TRKC; Mouse TRKC PicoKine ELISA Kit; Mouse TRKC; TRKC elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse MSTN
• Sequence Length: 839

• Samples: Cell Culture Supernates, Tissue, Serum And Plasma(Heparin Or Edta)
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TRKC elisa kit

Principle of the Assay: The Picokine Mouse MSTN Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse MSTN with a 96-well strip plate that is pre-coated with antibody specific for MSTN. The detection antibody is a biotinylated antibody specific for MSTN. The capture antibody is A monoclonal antibody from rat, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Mouse MSTN with immunogen: Expression system for standard: NSO, Immunogen sequence: D268-S376. The kit is analytically validated with ready to use reagents. To measure Mouse MSTN, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Mouse MSTN in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse MSTN in the sample!!Background/Introduction: Myostatin (also known as growth differentiation factor 8, abbreviated GDF-8) is a myokine, a protein produced and released by myocytes that acts on muscle cells' autocrine function to inhibit myogenesis: muscle cell growth and differentiation. In humans it is encoded by the MSTN gene. It is mapped to 1 C1.1; 1 26.99 cM. Myostatin is a secreted growth differentiation factor that is a member of the TGF beta protein family. This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGFbeta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Mutations in this gene are associated with increased skeletal muscle mass in humans and other mammals.

NCBI and Uniprot Product Information

• NCBI GI #: 3336860
• NCBI GeneID: 4916
• UniProt Accession #: Q16288
• NCBI Official Full Name: TRKC
• NCBI Official Synonym Full Names: neurotrophic receptor tyrosine kinase 3
• NCBI Official Symbol: NTRK3
• NCBI Official Synonym Symbols: TRKC; GP145-TrkC; gp145(trkC)
• NCBI Protein Information: NT-3 growth factor receptor

NCBI Description

This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. Mutations in this gene have been associated with medulloblastomas, secretory breast carcinomas and other cancers. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]