SDS-Page
(Peptide Competition: Extracts prepared from cardiomyocytes overexpressing mutant MBS614315 S105A (1) or wild-type MBS614315 (2-5) stimulated with phenylephrine (PE) for 24 hours were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4ºC, then were incubated with 0.35 ug/ml MBS614315 [pS105] antibody for two hours at RT in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2),)
Rabbit anti-Mouse GATA4 Polyclonal Antibody | anti-GATA4 antibody
GATA4, phosphorylated (Ser105) (GATA Binding Factor 4, GATA Binding Protein 4)
Purified by Immunoaffinity chromatography.
Purified by Immunoaffinity chromatography.
Dilution: Western Blot: 0.1-1ug/ml
SDS-Page
(Peptide Competition: Extracts prepared from cardiomyocytes overexpressing mutant MBS614315 S105A (1) or wild-type MBS614315 (2-5) stimulated with phenylephrine (PE) for 24 hours were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4ºC, then were incubated with 0.35 ug/ml MBS614315 [pS105] antibody for two hours at RT in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2),)
SDS-Page
(Peptide Competition: Extracts prepared from cardiomyocytes overexpressing mutant MBS614315 S105A (1) or wild-type MBS614315 (2-5) stimulated with phenylephrine (PE) for 24 hours were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4ºC, then were incubated with 0.35 ug/ml MBS614315 [pS105] antibody for two hours at RT in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2),)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects. [provided by RefSeq]
Uniprot Description
GATA4: a conserved and ubiquitous member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. May regulate a set of cardiac-specific genes and play a crucial role in cardiogenesis. Defects are a cause of atrial septal defect 2 (ASD2). ASD2 is an autosomal dominant condition with atrial septal defect and other congenital heart disease but no conduction defects or noncardiac abnormalities.
Protein type: Motility/polarity/chemotaxis; Transcription factor
Chromosomal Location of Human Ortholog: 8p23.1-p22
Cellular Component: nucleoplasm; nuclear chromatin; nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; NFAT protein binding; transcription activator binding; DNA binding; zinc ion binding; sequence-specific DNA binding; transcription coactivator activity; chromatin binding; transcription factor binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; cardiac muscle hypertrophy; negative regulation of autophagy; embryonic heart tube anterior/posterior pattern formation; positive regulation of transcription, DNA-dependent; Sertoli cell differentiation; Wnt receptor signaling pathway through beta-catenin; BMP signaling pathway; regulation of transcription, DNA-dependent; cell-cell signaling; embryonic digestive tract morphogenesis; embryonic foregut morphogenesis; heart looping; positive regulation of BMP signaling pathway; positive regulation of cardiac muscle cell proliferation; response to drug; response to retinoic acid; in utero embryonic development; male gonad development; gastrulation with mouth forming second; positive regulation of angiogenesis; response to estrogen stimulus; endoderm formation; response to mechanical stimulus; positive regulation of cardioblast differentiation; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; endoderm development
Disease: Ventricular Septal Defect 1; Atrial Septal Defect 2; Tetralogy Of Fallot; Atrioventricular Septal Defect 4; Testicular Anomalies With Or Without Congenital Heart Disease
Research Articles on GATA4
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Product Notes
The GATA4 gata4 (Catalog #AAA614315) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GATA4, phosphorylated (Ser105) (GATA Binding Factor 4, GATA Binding Protein 4) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GATA4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 0.1-1ug/ml. Researchers should empirically determine the suitability of the GATA4 gata4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GATA4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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