Rabbit anti-Human, Mouse BPI Polyclonal Antibody | anti-BPI antibody

BPI Antibody

Cat. Num. AAA9408215

• Gene Names: BPI; rBPI; BPIFD1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: BPI; Polyclonal Antibody; BPI Antibody; rBPI; BPIFD1; anti-BPI antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total BPI protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 487

• Applicable Applications for anti-BPI antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:200; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human BPI.
• Target Name: BPI
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using BPI antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse lung using BPI antibody at dilution of 1:200 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse liver cancer using BPI antibody at dilution of 1:200 (400x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of A549 cell using BPI antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-BPI antibody

This gene encodes a lipopolysaccharide binding protein. It is associated with human neutrophil granules and has bactericidal activity on gram-negative organisms.

Product Categories/Family for anti-BPI antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 157276599
• NCBI GeneID: 671
• NCBI Accession #: NP_001716.2
• NCBI GenBank Nucleotide #: NM_001725.2
• UniProt Accession #: P17213; Q1ZZU8; Q5JRW0; Q8IW58; Q9BYZ9; Q9H1L2; Q9H1M8; Q9H203; Q9UCT4; Q9UD65; B2RCY2
• Molecular Weight: 53,900 Da
• NCBI Official Full Name: bactericidal permeability-increasing protein
• NCBI Official Synonym Full Names: bactericidal/permeability-increasing protein
• NCBI Official Symbol: BPI
• NCBI Official Synonym Symbols: rBPI; BPIFD1
• NCBI Protein Information: bactericidal permeability-increasing protein
• UniProt Protein Name: Bactericidal permeability-increasing protein
• Protein Family: BPI fold-containing family
• UniProt Gene Name: BPI
• UniProt Synonym Gene Names: BPI
• UniProt Entry Name: BPI_HUMAN

NCBI Description

This gene encodes a lipopolysaccharide binding protein. It is associated with human neutrophil granules and has antimicrobial activity against gram-negative organisms. [provided by RefSeq, Nov 2014]

Uniprot Description

BPI: The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.

Protein type: Secreted, signal peptide; Cell surface; Secreted

Chromosomal Location of Human Ortholog: 20q11.23

Cellular Component: integral to plasma membrane; cytoplasm

Molecular Function: lipopolysaccharide binding

Biological Process: negative regulation of interleukin-6 production; negative regulation of interleukin-8 production; immune response; negative regulation of tumor necrosis factor production; defense response to Gram-negative bacterium; negative regulation of macrophage activation