Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Eotaxin 1 (CCL11) Standard Curve.)
Rat Eotaxin 1 (CCL11) ELISA Kit | CCL11 elisa kit
Rat Eotaxin 1 (CCL11) ELISA Kit
Gene Names
Ccl11; Scya11
Reactivity
Rat
Synonyms
Eotaxin 1 (CCL11); Rat Eotaxin 1 (CCL11) ELISA Kit; CCL11; MGC22554; SCYA11; eosinophil chemotactic protein; eotaxin-1; small inducible cytokine A11; small inducible cytokine subfamily A (Cys-Cys); member 11 (eotaxin); ECF; CCL11 elisa kit
Reactivity
Rat
Specificity
Rat Eotaxin 1 (CCL11) ELISA Kit has high sensitivity and excellent specificity for detection of Rat CCL11. No significant cross-reactivity or interference between Rat CCL11 and analogues was observed.
Sequence Length
97
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Eotaxin 1 (CCL11) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Eotaxin 1 (CCL11) Standard Curve.)
Related Product Information for CCL11 elisa kit
Description: This Rat Eotaxin 1 (CCL11) ELISA Kit employs a two-site sandwich ELISA to quantitate CCL11 in samples. An antibody specific for CCL11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCL11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL11 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL11 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: An eosinophil chemotactic factor (ECF) can be released from human polymorphonuclear neutrophils (PMN), rat mononuclear and rat mast cells by the calcium ionophore, during phagocytosis, by arachidonic acid and phospholipase A2. It has been suggested that stimuli such as the ionophore and the phagocytic event lead to phospholipid turnover with the generation of arachidonic acid which is subsequently transformed by a lipoxygenase-like enzyme into ECF. Chemokine (C-C motif) ligand 11 (CCL11) is a small cytokine belonging to the CC chemokine family that is also known as eotaxin-1. CCL11 selectively recruits eosinophils by inducing their chemotaxis, and therefore, is implicated in allergic responses.
Background: An eosinophil chemotactic factor (ECF) can be released from human polymorphonuclear neutrophils (PMN), rat mononuclear and rat mast cells by the calcium ionophore, during phagocytosis, by arachidonic acid and phospholipase A2. It has been suggested that stimuli such as the ionophore and the phagocytic event lead to phospholipid turnover with the generation of arachidonic acid which is subsequently transformed by a lipoxygenase-like enzyme into ECF. Chemokine (C-C motif) ligand 11 (CCL11) is a small cytokine belonging to the CC chemokine family that is also known as eotaxin-1. CCL11 selectively recruits eosinophils by inducing their chemotaxis, and therefore, is implicated in allergic responses.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
eotaxin
NCBI Official Synonym Full Names
C-C motif chemokine ligand 11
NCBI Official Symbol
Ccl11
NCBI Official Synonym Symbols
Scya11
NCBI Protein Information
eotaxin
UniProt Protein Name
Eotaxin
Protein Family
UniProt Gene Name
Ccl11
UniProt Synonym Gene Names
Scya11
UniProt Entry Name
CCL11_RAT
NCBI Description
mouse homolog is an eosinophil chemoattractant [RGD, Feb 2006]
Uniprot Description
In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions.
Research Articles on CCL11
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Product Notes
The Rat CCL11 ccl11 (Catalog #AAA9712696) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712696 ELISA Kit recognizes Rat CCL11. It is sometimes possible for the material contained within the vial of "Eotaxin 1 (CCL11), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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