Rat CCL11/Eotaxin PicoKine ELISA Kit | Ccl11 elisa kit

Rat CCL11/Eotaxin PicoKine ELISA Kit

Cat. Num. AAA177626

• Gene Names: Ccl11; Scya11
• Reactivity: Rat
• Synonyms: CCL11/Eotaxin PicoKine; Rat CCL11/Eotaxin PicoKine ELISA Kit; Eotaxin; C-C motif chemokine 11; CCL 11; CCL11; CCL11_HUMAN; Chemokine (C-C motif) ligand 11; Chemokine C C motif ligand 11; Chemokine CC Motif Ligand 11; Chemokine ligand 11; Eosinophil Chemotactic P; Eosinophil chemotactic protein; Eotaxin 1; SCYA 11; SCYA11; chemokine (C-C motif) ligand 11; Ccl11 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant rat Eotaxin
• Sequence Length: 97

• Assay Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of rat Eotaxin in cell culture supernates, serum and plasma(EDTA, citrate).
• Immunogen: E Coli, H24-P97
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml rat Eotaxin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernatants, serum or plasma(EDTA, citrate) to each empty well. It is recommended that each rat Eotaxin standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|74.6|267|646|101.4|309|718; Standard deviation|3.80|14.55|36.18|6.49|22.25|56.72; CV(%)|5.1|6.2|5.6|6.4|7.2|7.9
• Sample Data: Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000; O.D.|0.015|0.104|0.151|0.286|0.579|1.050|2.051|2.562
• Anticoagulant: EDTA or Citrate
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Rat Eotaxin PicoKine ELISA Kit standard curve )

Related Product Information for Ccl11 elisa kit

Principle of the assay: Rat Eotaxin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Eotaxin has been precoated onto 96-well plates. Standards(Expression system for standard: E Coli, Immunogen sequence: H24-P97) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Eotaxin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Eotaxin amount of sample captured in plate.

Background: Eotaxin, also known as CCL11, is a potent inducer of eosinophil chemotaxis and is considered as a selective ligand of the CC chemokine receptor 3 (CCR3), which is expressed on eosinophils, basophils, and Th2 lymphocytes. Eotaxin is assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections. The gene maps to chromosome 17 and is expressed constitutively at high levels in small intestine and colon, and at lower levels in various other tissues. The deduced mature protein sequence is 66% identical to human monocyte chemoattractant protein-1, and 60% identical to guinea pig eotaxin.

NCBI and Uniprot Product Information

• NCBI GI #: 11276089
• NCBI GeneID: 29397
• NCBI Accession #: NP_062078.1
• NCBI GenBank Nucleotide #: NM_019205.1
• UniProt Accession #: P97545; O08780
• Molecular Weight: 10,851 Da
• NCBI Official Full Name: eotaxin
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 11
• NCBI Official Symbol: Ccl11
• NCBI Official Synonym Symbols: Scya11
• NCBI Protein Information: eotaxin
• UniProt Protein Name: Eotaxin
• Protein Family: Eotaxin
• UniProt Gene Name: Ccl11
• UniProt Synonym Gene Names: Scya11
• UniProt Entry Name: CCL11_RAT

NCBI Description

mouse homolog is an eosinophil chemoattractant [RGD, Feb 2006]

Uniprot Description

In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions.