Typical Testing Data/Standard Curve (for reference only)
Rat Eotaxin 1 ELISA Kit | Eotaxin 1 elisa kit
Rat Eotaxin 1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This EOTAXIN 1 ELISA kit is intended for laboratory research use only. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of EOTAXIN 1 in the sample, this EOTAXIN 1 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus EOTAXIN 1 concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of EOTAXIN 1 utilizes a multiclonal anti-EOTAXIN 1 antibody and an EOTAXIN 1-HRP conjugate. The assay sample and buffer are incubated together with EOTAXIN 1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EOTAXIN 1 concentration since EOTAXIN 1 from samples and EOTAXIN 1-HRP conjugate compete for the anti-EOTAXIN 1 antibody binding site. Since the number of sites is limited, as more sites are occupied by EOTAXIN 1 from the sample, fewer sites are left to bind EOTAXIN 1-HRP conjugate. Standards of known EOTAXIN 1 concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of EOTAXIN 1. The EOTAXIN 1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, displays chemotactic activity for eosinophils, but not mononuclear cells or neutrophils. This eosinophil-specific chemokine is thought to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections. [provided by RefSeq, Jul 2013]
Uniprot Description
Function: In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3.
Subcellular location: Secreted.
Induction: By TNF, IL1A/interleukin-1 alpha and IFNG/IFN-gamma.
Post-translational modification: O-linked glycan consists of a Gal-GalNAc disaccharide which is mofified with up to 2 sialic acid residues.
Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.
Research Articles on Eotaxin 1
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Product Notes
The Rat Eotaxin 1 ccl11 (Catalog #AAA722247) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA722247 ELISA Kit recognizes Rat Eotaxin 1. It is sometimes possible for the material contained within the vial of "Eotaxin 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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