Typical Testing Data/Standard Curve (for reference only)
(Mouse Eotaxin PicoKine ELISA Kit standard curve )
Mouse Eotaxin ELISA Kit | CCL11 elisa kit
Mouse Eotaxin PicoKine ELISA Kit
Gene Names
Ccl11; Scya11; eotaxin
Reactivity
Mouse
Synonyms
Eotaxin; Mouse Eotaxin PicoKine ELISA Kit; chemokine (C-C motif) ligand 11; C-C motif chemokine 11; CCL 11; CCL11; CCL11_HUMAN; Chemokine (C-C motif) ligand 11; Chemokine C C motif ligand 11; Chemokine CC Motif Ligand 11; Chemokine ligand 11; Eosinophil Chemotactic P; Eosinophil chemotactic protein; Eotaxin 1; MGC22554; SCYA 11; SCYA11; Small inducible cytokine A11; Small inducible cytokine subfamily A (Cys Cys) member 11; Small inducible cytokine subfamily A member 11; Small-inducible cytokine A11; CCL11 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse Eotaxin
Sequence Length
97
Samples
Cell culture supernates, serum and plasma (citrate, EDTA)
Assay Type
Sandwich
Detection Range
15.6pg/ml-1000pg/ml
Sensitivity
< 1pg/ml
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Mouse Eotaxin PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse Eotaxin PicoKine ELISA Kit standard curve )
Related Product Information for CCL11 elisa kit
Principle of the assay: mouse Eotaxin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Eotaxin has been precoated onto 96-well plates. Standards(E.coli,H24-P97) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Eotaxin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Eotaxin amount of sample captured in plate.
Background: Eotaxin, also known as CCL11, is a potent inducer of eosinophil chemotaxis and is considered as a selective ligand of the CC chemokine receptor 3 (CCR3), which is expressed on eosinophils, basophils, and Th2 lymphocytes.1Eotaxin is assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections.2 The gene maps to chromosome 17 and is expressed constitutively at high levels insmall intestine and colon, and at lower levels in various other tissues. The deduced mature protein sequence is 66% identical to human monocyte chemoattractant protein-1, and 60% identical to guinea pig eotaxin.
Background: Eotaxin, also known as CCL11, is a potent inducer of eosinophil chemotaxis and is considered as a selective ligand of the CC chemokine receptor 3 (CCR3), which is expressed on eosinophils, basophils, and Th2 lymphocytes.1Eotaxin is assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections.2 The gene maps to chromosome 17 and is expressed constitutively at high levels insmall intestine and colon, and at lower levels in various other tissues. The deduced mature protein sequence is 66% identical to human monocyte chemoattractant protein-1, and 60% identical to guinea pig eotaxin.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
10,893 Da
NCBI Official Full Name
Eotaxin
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 11
NCBI Official Symbol
Ccl11
NCBI Official Synonym Symbols
Scya11; eotaxin
NCBI Protein Information
eotaxin; C-C motif chemokine 11; small inducible cytokine A11; small-inducible cytokine A11; eosinophil chemotactic protein; small chemokine (C-C motif) ligand 11
UniProt Protein Name
Eotaxin
Protein Family
UniProt Gene Name
Ccl11
UniProt Synonym Gene Names
Scya11
UniProt Entry Name
CCL11_MOUSE
Uniprot Description
CCL11: In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide
Cellular Component: extracellular space; extracellular region; intracellular
Molecular Function: chemokine activity; cytokine activity
Biological Process: regulation of cell shape; positive regulation of angiogenesis; positive regulation of actin filament polymerization; mast cell chemotaxis; eosinophil chemotaxis; actin filament organization; positive regulation of endothelial cell proliferation; immune response; cytoskeleton organization and biogenesis; chemotaxis; inflammatory response; positive regulation of cell migration
Research Articles on CCL11
Similar Products
Product Notes
The Mouse CCL11 ccl11 (Catalog #AAA175964) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA175964 ELISA Kit recognizes Mouse CCL11. It is sometimes possible for the material contained within the vial of "Eotaxin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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