EGF recombinant protein

Rat EGF protein

Cat. Num. AAA9719015

• Purity: >=98% as determined by SDS-PAGE
• Synonyms: EGF; Rat EGF protein; EGF recombinant protein

• Host: E Coli
• Purity/Purification: >=98% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 50mM NaCl pH9.5.
• Sequence: Asn974-Arg1026

• Protein Construction: Amino acid sequence derived from Rat EGF isoform (P07522)(Asn974-Arg1026) was expressed.
• Protein Length: The recombinant Rat EGF consists of 53 amino acids and has a predicted molecular mass of 6.3kDa. The apparent molecular mass of the Rat EGF protein is approximately 6.3kDa in SDS-PAGE under reducing conditions.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to centrifuge at 12,000 rpm for 1 minute.Then reconstitute the lyophilized Rat EGF protein using the buffer we provided not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Lyophilized Rat EGF protein product should be stored desiccated below -18 degree C for est. 12 months. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and below -18 degree C for est. 3 months. For long term storage it is recommended to add a carrier protein (example 0.1% HSA or BSA), aliquot and store below -18 degree C. Please prevent freeze-thaw cycles.

SDS-Page

(Fig.SDS-PAGE analysis of Rat EGF protein)

Related Product Information for EGF recombinant protein

Description: Recombinant Rat EGF protein expressed in E Coli.
Background: EGF is a single-pass type I membrane protein,containing 8 LDL-receptor class B repeats and 9 EGF-like domains. EGF is a growth factor that stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. It results in cellular proliferation, differentiation, and survival.Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-alpha and VGF (vaccinia virus growth factor).

Product Categories/Family for EGF recombinant protein

Cell Culture; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 6978797
• NCBI GeneID: 25313
• NCBI Accession #: NP_036974.1
• NCBI GenBank Nucleotide #: NM_012842.1
• UniProt Accession #: P07522; Q63183
• Molecular Weight: 124,126 Da
• NCBI Official Full Name: pro-epidermal growth factor
• NCBI Official Synonym Full Names: epidermal growth factor
• NCBI Official Symbol: Egf
• NCBI Protein Information: pro-epidermal growth factor
• UniProt Protein Name: Pro-epidermal growth factor
• Protein Family: EGF-like domain containing protein
• UniProt Gene Name: Egf
• UniProt Synonym Gene Names: EGF
• UniProt Entry Name: EGF_RAT

NCBI Description

binds the epidermal growth factor receptor; may play a role in MAP kinase mediated signaling pathways [RGD, Feb 2006]

Uniprot Description

EGF: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4); also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligand, receptor tyrosine kinase; Hormone; Membrane protein, integral

Cellular Component: extracellular space; lysosomal membrane; plasma membrane; integral to membrane; intracellular

Molecular Function: growth factor activity; calcium ion binding; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; peptidyl-tyrosine phosphorylation; activation of MAPKK activity; positive regulation of mitosis; positive regulation of transcription, DNA-dependent; regulation of protein transport; positive regulation of MAP kinase activity; regulation of protein secretion; branching morphogenesis of a tube; positive regulation of epidermal growth factor receptor activity; positive regulation of cell proliferation; negative regulation of secretion; angiogenesis; regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphorylation; STAT protein nuclear translocation; positive regulation of DNA binding; positive regulation of granule cell precursor proliferation