EGF active protein

Rat EGF

Cat. Num. AAA695954

• Reactivity: Species Reactivity: Rat; Cross Reactivity: Mouse
• Purity: Above 98% as determined by SDS-PAGE Analysis.
• Synonyms: EGF; Rat EGF; Recombinant Rat EGF; EGF active protein

• Host: E Coli
• Reactivity: Species Reactivity: Rat; Cross Reactivity: Mouse
• Purity/Purification: Above 98% as determined by SDS-PAGE Analysis.
• Sequence: MNSNTGCPP SYDGYCLNG GVCMYVESV DRYVCNCVI GYIGERCQH RDLRWWKLR

• Biological Activity: The ED50 was determined by a cell proliferation assay using BALB/c 3T3 cells is <= 0.1 ng/ml, corresponding to a specific activity of >= 1 x 107 units/mg.
• Preparation and Storage: At -20 degree C

Testing Data

Related Product Information for EGF active protein

EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF- alpha and VGF (vaccinia virus growth factor). Recombinant rat EGF is a 6.2 kDa globular protein containing 54 amino acid residues including 3 intramolecular disulfide-bonds.

Product Categories/Family for EGF active protein

Stem Cell Research; Cancer; Angiogenesis; Neurosciences; Inflammation; Proliferation and Cell Cycle

NCBI and Uniprot Product Information

• NCBI GI #: 1352360
• NCBI GeneID: 25313
• UniProt Accession #: P07522; Q63183
• Molecular Weight: 124,126 Da
• NCBI Official Full Name: Pro-epidermal growth factor
• NCBI Official Synonym Full Names: epidermal growth factor
• NCBI Official Symbol: Egf
• NCBI Protein Information: pro-epidermal growth factor
• UniProt Protein Name: Pro-epidermal growth factor
• Protein Family: EGF-like domain containing protein
• UniProt Gene Name: Egf
• UniProt Synonym Gene Names: EGF
• UniProt Entry Name: EGF_RAT

NCBI Description

binds the epidermal growth factor receptor; may play a role in MAP kinase mediated signaling pathways [RGD, Feb 2006]

Uniprot Description

EGF: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4); also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligand, receptor tyrosine kinase; Hormone; Membrane protein, integral

Cellular Component: extracellular space; lysosomal membrane; integral to membrane; plasma membrane; intracellular

Molecular Function: growth factor activity; calcium ion binding; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; peptidyl-tyrosine phosphorylation; positive regulation of mitosis; activation of MAPKK activity; positive regulation of transcription, DNA-dependent; regulation of protein transport; positive regulation of MAP kinase activity; regulation of protein secretion; branching morphogenesis of a tube; positive regulation of epidermal growth factor receptor activity; positive regulation of cell proliferation; negative regulation of secretion; angiogenesis; regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphorylation; positive regulation of DNA binding; STAT protein nuclear translocation; positive regulation of granule cell precursor proliferation