Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat DNA topoisomerase 1 (TOP1) Standard Curve.)
Rat DNA Topoisomerase 1 (TOP1) ELISA Kit | TOP1 elisa kit
Rat DNA Topoisomerase 1 (TOP1) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat DNA topoisomerase 1 (TOP1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat DNA topoisomerase 1 (TOP1) Standard Curve.)
Background: DNA topoisomerase 1 isa DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.Type IA topoisomerases creates a single break in DNA, and passes a second strand or duplex through the break. This strand passage mechanism shares several features with type IIA topoisomerases. They both form a 5' phosphotyrosine intermediate, and require a divalent metal ion to perform its work. Unlike type II topoisomerases, type IA topoisomerases do not use energy to do its work
NCBI and Uniprot Product Information
NCBI Description
human homolog catalyzes the transient breakage and rejoining of DNA; plays a role in nucleotide excision repair [RGD, Feb 2006]
Uniprot Description
TOP1: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98. Belongs to the type IB topoisomerase family.
Protein type: EC 5.99.1.2; Isomerase; Nucleolus; Oncoprotein
Chromosomal Location of Human Ortholog: 3q42
Cellular Component: cytoplasm; dense fibrillar component; DNA topoisomerase complex (ATP-hydrolyzing); fibrillar center; nuclear chromosome; nucleolus; nucleoplasm; nucleus; perikaryon
Molecular Function: ATP binding; chromatin binding; chromatin DNA binding; DNA binding; DNA topoisomerase type I activity; double-stranded DNA binding; protein complex binding; protein domain specific binding; protein serine/threonine kinase activity; RNA binding; single-stranded DNA binding
Biological Process: chromatin remodeling; chromosome segregation; circadian regulation of gene expression; circadian rhythm; DNA replication; DNA topological change; embryonic cleavage; organ regeneration; peptidyl-serine phosphorylation; response to cAMP; response to drug; response to gamma radiation; response to temperature stimulus; rRNA transcription
Research Articles on TOP1
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Product Notes
The Rat TOP1 top1 (Catalog #AAA9712536) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712536 ELISA Kit recognizes Rat TOP1. It is sometimes possible for the material contained within the vial of "DNA Topoisomerase 1 (TOP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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