DUSP18 active protein

DUSP18, human recombinant

Cat. Num. AAA844447

• Gene Names: DUSP18; DSP18; DUSP20; LMWDSP20
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: DUSP18; human recombinant; Dual specificity protein phosphatase 18; DUSP20; DUSP18 active protein

• Host: E Coli
• Purity/Purification: >=95%
• Form/Format: 0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.1 mM PMSF, 1 mM EDTA and 1 mM DTT; Appearance: Liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMTAPSC AFPVQFRQPS VSGLSQITKS LYISNGVAAN NKLMLSSNQI TMVINVSVEV VNTLYEDIQY MQVPVADSPN SRLCDFFDPI ADHIHSVEMK QGRTLLHCAA GVSRSAALCL AYLMKYHAMS LLDAHTWTKS CRPIIRPNSG FWEQLIHYEF QLFGKNTVHM VSSPVGMIPD IYEKEVRLMI PL
• Sequence Length: 188

• Applicable Applications for DUSP18 active protein: SDS-PAGE
• Biological Activity: Specific activity: >1,100 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37 degree C, pH 7.5 using 10 mM of substrate
• Results: Specific activity: >1,100 units/mg
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for DUSP18 active protein

Background: Dual specificity phosphatase 18, also known as DuSP18, is a member of the dual-specificity phosphatase (DSP) family, which catalyzes dephosphorylation of phosphotyrosine and phosphothreonine residues. DuSP18 is inhibited by iodoarectic acid and is activated by manganese ions. Along with having preferential enzymatic activity against phosphorylated tyrosine residues over threonine residues, DuSP18 also dephosphorylates p-nitrophenyl phosphate (pNPP) in vitro. This protein is widely expressed with highest levels in liver, brain, ovary and testis. Recombinant human DuSP18 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Product Categories/Family for DUSP18 active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 755571545
• NCBI GeneID: 150290
• NCBI Accession #: NP_001291723.1
• NCBI GenBank Nucleotide #: NM_001304794.1
• UniProt Accession #: Q8NEJ0; B3KPA4
• Molecular Weight: 40.4 kDa (359 aa, 149-482 aa + His Tag)
• NCBI Official Full Name: dual specificity protein phosphatase 18 isoform a
• NCBI Official Synonym Full Names: dual specificity phosphatase 18
• NCBI Official Symbol: DUSP18
• NCBI Official Synonym Symbols: DSP18; DUSP20; LMWDSP20
• NCBI Protein Information: dual specificity protein phosphatase 18
• UniProt Protein Name: Dual specificity protein phosphatase 18
• Protein Family: Dual specificity protein phosphatase
• UniProt Gene Name: DUSP18
• UniProt Synonym Gene Names: LMWDSP20; LMW-DSP20
• UniProt Entry Name: DUS18_HUMAN

NCBI Description

Dual-specificity phosphatases (DUSPs) constitute a large heterogeneous subgroup of the type I cysteine-based protein-tyrosine phosphatase superfamily. DUSPs are characterized by their ability to dephosphorylate both tyrosine and serine/threonine residues. They have been implicated as major modulators of critical signaling pathways. DUSP18 contains the consensus DUSP C-terminal catalytic domain but lacks the N-terminal CH2 domain found in the MKP (mitogen-activated protein kinase phosphatase) class of DUSPs (see MIM 600714) (summary by Patterson et al., 2009 [PubMed 19228121]).[supplied by OMIM, Dec 2009]

Uniprot Description

DUSP18: Can dephosphorylate single and diphosphorylated synthetic MAPK peptides, with preference for the phosphotyrosine and diphosphorylated forms over phosphothreonine. In vitro, dephosphorylates p-nitrophenyl phosphate (pNPP). Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.

Protein type: Motility/polarity/chemotaxis; EC 3.1.3.48; EC 3.1.3.16; Mitochondrial; Protein phosphatase, dual-specificity

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity; MAP kinase tyrosine/serine/threonine phosphatase activity

Biological Process: inactivation of MAPK activity