Rabbit anti-Human, Mouse ErbB3 Polyclonal Antibody | anti-ERBB3 antibody

ErbB3, NT (Receptor Tyrosine-protein Kinase erbB-3, Proto-oncogene-like Protein c-ErbB-3, Tyrosine Kinase-type Cell Surface Receptor HER3, HER3)

Cat. Num. AAA6009408

• Gene Names: ERBB3; HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: ErbB3; Polyclonal Antibody; NT (Receptor Tyrosine-protein Kinase erbB-3; Proto-oncogene-like Protein c-ErbB-3; Tyrosine Kinase-type Cell Surface Receptor HER3; HER3); Anti -ErbB3; anti-ERBB3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ErbB3. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-ERBB3 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in ELISA, Western Blot, Flow Cytometry, and Immunohistochemistry.; Dilution: Western Blot: 1:100-1:500; ELISA: 1:1,000; Immunohistochemistry: 1:50-1:100; Flow Cytometry: 1:10-1:50
• Immunogen: Synthetic peptide selected from the N-terminal region of human ErbB3 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse brain lysate using MBS6009408. ErbB3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human breast carcinoma using MBS6009408, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Testing Data

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of 293 cells using MBS6009408 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ERBB3 antibody

ErbB3, a member of the EGF receptor family, binds and is activated by neuregulins and NTAK. It potentially forms a heterodimer with each of the other ERBB receptors. This protein is predominantly expressed in epithelial tissues and brain. The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins. Ligand-binding may increase phosphorylation on tyrosine residues and promote its association with the p85 subunit of phosphatidylinositol 3-kinase ErbB3 is overexpressed in a subset of human mammary tumors.

Product Categories/Family for anti-ERBB3 antibody

Antibodies; Abs to Oncoproteins

NCBI and Uniprot Product Information

• NCBI GI #: 12803739
• NCBI GeneID: 2065
• UniProt Accession #: P21860; Q9BUD7; A8K6L6; B4DIK7; B4DV32; E9PDT8
• Molecular Weight: 148,098 Da
• NCBI Official Full Name: ERBB3 protein
• NCBI Official Synonym Full Names: v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3
• NCBI Official Symbol: ERBB3
• NCBI Official Synonym Symbols: HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3
• NCBI Protein Information: receptor tyrosine-protein kinase erbB-3; proto-oncogene-like protein c-ErbB-3; tyrosine kinase-type cell surface receptor HER3
• UniProt Protein Name: Receptor tyrosine-protein kinase erbB-3
• Protein Family: Receptor tyrosine-protein kinase
• UniProt Gene Name: ERBB3
• UniProt Synonym Gene Names: HER3
• UniProt Entry Name: ERBB3_HUMAN

NCBI Description

This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

HER3: a receptor tyrosine kinase of the EGFR family. Binds and is activated by neuregulins and NTAK. Can form homodimers or ErbB-2/ErbB-3 heterodimers. Kinase domain lacks activity but heterodimerizes with other EGFRs to transduce growth signals. May be required for HER2 activity. Elevated expression in breast and other tumors is indicative of poor outcome. A secreted form is expressed in metastatic prostate cancer Two alternatively spliced isoforms have been described.

Protein type: Membrane protein, integral; Oncoprotein; Protein kinase, TK; Kinase, protein; Protein kinase, tyrosine (receptor); EC 2.7.10.1; TK group; EGFR family

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: extracellular space; integral to plasma membrane; basolateral plasma membrane; apical plasma membrane; plasma membrane; lateral plasma membrane; receptor complex

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; transmembrane receptor activity; protein heterodimerization activity; protein-tyrosine kinase activity; growth factor binding; protein tyrosine kinase activator activity; ATP binding

Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide 3-kinase cascade; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; wound healing; nerve growth factor receptor signaling pathway; heart development; cranial nerve development; negative regulation of cell adhesion; signal transduction; protein amino acid phosphorylation; negative regulation of signal transduction; peripheral nervous system development; regulation of cell proliferation; positive regulation of phosphoinositide 3-kinase cascade; Schwann cell differentiation; neuron apoptosis; innate immune response; negative regulation of secretion; negative regulation of neuron apoptosis; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Lethal Congenital Contracture Syndrome 2