Western Blot (WB)
(Western Blot analysis in Hela whole cell lysate (20ug/lane) using MBS642928 (1:1000). Secondary IgG, (H+L) (HRP) goat anti-rabbit at 1:10,000 dilution. Predicted band size: 192kD Blocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human CLTC Polyclonal Antibody | anti-CLTC antibody
CLTC, ID (CLTC, CLH17, CLTCL2, KIAA0034, Clathrin heavy chain 1, Clathrin heavy chain on chromosome 17) (MaxLight 750)
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western Blot analysis in Hela whole cell lysate (20ug/lane) using MBS642928 (1:1000). Secondary IgG, (H+L) (HRP) goat anti-rabbit at 1:10,000 dilution. Predicted band size: 192kD Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Western Blot analysis in Hela whole cell lysate (20ug/lane) using MBS642928 (1:1000). Secondary IgG, (H+L) (HRP) goat anti-rabbit at 1:10,000 dilution. Predicted band size: 192kD Blocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using MBS642928 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS642928 for immunohistochemistry.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using MBS642928 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS642928 for immunohistochemistry.)
Flow Cytometry (FC/FACS)
(Flow Cytometry analysis of Hela cells using MBS642928 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow Cytometry analysis of Hela cells using MBS642928 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. [provided by RefSeq, Jul 2008]
Uniprot Description
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge (PubMed:15858577, PubMed:16968737, PubMed:21297582). The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension (PubMed:23532825). Plays a role in early autophagosome formation (PubMed:20639872).
Research Articles on CLTC
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Product Notes
The CLTC cltc (Catalog #AAA6286985) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CLTC, ID (CLTC, CLH17, CLTCL2, KIAA0034, Clathrin heavy chain 1, Clathrin heavy chain on chromosome 17) (MaxLight 750) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CLTC can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CLTC cltc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CLTC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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