Flow Cytometry (FC/FACS)
(Mouse abdominal macrophages elicited by starch broth are stained with Anti-Mouse F4/80 Monoclonal Antibody(PerCP/Cyanine5.5 Conjugated)(filled gray curve). Unstained macrophages (blank black curve) are used as control.)
Rat anti-Mouse F4/80 Monoclonal Antibody | anti-F4/80 antibody
Anti-Mouse F4/80 Monoclonal Antibody (PerCP/Cyanine5.5 Conjugated) [CI:A3-1]
Gene Names
Adgre1; Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Synonyms
F4/80; Monoclonal Antibody; Anti-Mouse F4/80 Monoclonal Antibody (PerCP/Cyanine5.5 Conjugated) [CI:A3-1]; Adhesion G protein-coupled receptor E1; Adgre1; Cell surface glycoprotein F4/80; EGF-like module receptor 1; Emr1; Gpf480; anti-F4/80 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
CI:A3-1
Form/Format
PBS with 0.05% Proclin300, 1% BSA
Concentration
0.5 mg/mL (varies by lot)
Sequence Length
931
Applicable Applications for anti-F4/80 antibody
Flow Cytometry (FC/FACS)
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is <= 2 ug per 106 cells in 100 ul volume or 100 ul of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Conjugation
PErCP/Cyanine5.5
Preparation and Storage
Store at 2-8 degree C and protect from prolonged exposure to light. Do not freeze.
Flow Cytometry (FC/FACS)
(Mouse abdominal macrophages elicited by starch broth are stained with Anti-Mouse F4/80 Monoclonal Antibody(PerCP/Cyanine5.5 Conjugated)(filled gray curve). Unstained macrophages (blank black curve) are used as control.)
Flow Cytometry (FC/FACS)
(Mouse abdominal macrophages elicited by starch broth are stained with Anti-Mouse F4/80 Monoclonal Antibody(PerCP/Cyanine5.5 Conjugated)(filled gray curve). Unstained macrophages (blank black curve) are used as control.)
Related Product Information for anti-F4/80 antibody
F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Adhesion G protein-coupled receptor E1
NCBI Official Synonym Full Names
adhesion G protein-coupled receptor E1
NCBI Official Symbol
Adgre1
NCBI Official Synonym Symbols
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
NCBI Protein Information
adhesion G protein-coupled receptor E1
UniProt Protein Name
EGF-like module-containing mucin-like hormone receptor-like 1
UniProt Gene Name
Emr1
UniProt Synonym Gene Names
Gpf480
UniProt Entry Name
EMR1_MOUSE
Uniprot Description
EMR1: Could be involved in cell-cell interactions. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 2
Cellular Component: membrane; integral to membrane; plasma membrane; external side of plasma membrane
Molecular Function: G-protein coupled receptor activity; signal transducer activity; transmembrane receptor activity; calcium ion binding
Biological Process: G-protein coupled receptor protein signaling pathway; cell surface receptor linked signal transduction; signal transduction
Research Articles on F4/80
Similar Products
Product Notes
The F4/80 emr1 (Catalog #AAA2558009) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mouse F4/80 Monoclonal Antibody (PerCP/Cyanine5.5 Conjugated) [CI:A3-1] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's F4/80 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is <= 2 ug per 106 cells in 100 ul volume or 100 ul of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Researchers should empirically determine the suitability of the F4/80 emr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "F4/80, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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