Human C-C motif chemokine 7 ELISA Kit | CCL7 elisa kit

Human C-C motif chemokine 7 ELISA Kit

Cat. Num. AAA764652

• Gene Names: CCL7; FIC; MARC; MCP3; NC28; MCP-3; SCYA6; SCYA7
• Reactivity: Human
• Synonyms: C-C motif chemokine 7; Human C-C motif chemokine 7 ELISA Kit; CCL7/MCP-3/MCP3/FIC/MARC/NC28/SCYA6/SCYA7; CCL7 elisa kit

• Reactivity: Human
• Sequence Length: 99

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.6-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CCL7 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CCL7 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CCL7 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CCL7 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CCL7 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 13435402
• NCBI GeneID: 6354
• NCBI Accession #: NP_006264.2
• NCBI GenBank Nucleotide #: NM_006273.3
• Molecular Weight: 11,200 Da
• NCBI Official Full Name: C-C motif chemokine 7
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 7
• NCBI Official Symbol: CCL7
• NCBI Official Synonym Symbols: FIC; MARC; MCP3; NC28; MCP-3; SCYA6; SCYA7
• NCBI Protein Information: C-C motif chemokine 7
• UniProt Protein Name: C-C motif chemokine 7
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL7
• UniProt Synonym Gene Names: MCP3; SCYA6; SCYA7; MCP-3
• UniProt Entry Name: CCL7_HUMAN

NCBI Description

This gene encodes monocyte chemotactic protein 3, a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix. This gene is part of a cluster of C-C chemokine family members on chromosome 17q. [provided by RefSeq, Jul 2008]

Uniprot Description

CCL7: Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17q11.2-q12

Cellular Component: cell; extracellular region; extracellular space

Molecular Function: CCR1 chemokine receptor binding; CCR2 chemokine receptor binding; chemokine activity; heparin binding; protein binding

Biological Process: cell-cell signaling; cellular calcium ion homeostasis; chemotaxis; cytoskeleton organization and biogenesis; eosinophil chemotaxis; G-protein coupled receptor protein signaling pathway; inflammatory response; lymphocyte chemotaxis; monocyte chemotaxis; neutrophil chemotaxis; positive regulation of cell migration; positive regulation of GTPase activity; positive regulation of inflammatory response; regulation of cell shape; response to gamma radiation; signal transduction