Typical Testing Data/Standard Curve (for reference only)
(Rat BTC PicoKine ELISA Kit standard curve )
Rat BTC ELISA Kit | BTC elisa kit
Rat BTC PicoKine ELISA Kit
Reactivity
Rat
Synonyms
BTC; Rat BTC PicoKine ELISA Kit; Probetacellulin; betacellulin; Betacellulin precursor; BTC elisa kit
Reactivity
Rat
Specificity
Natural and recombinant rat BTC
Sequence Length
177
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
7.8pg/ml-500pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Rat BTC PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Rat BTC PicoKine ELISA Kit standard curve )
Related Product Information for BTC elisa kit
Principle of the assay: rat BTC ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BTC has been precoated onto 96-well plates. Standards(E.coli, D32-Y111) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BTC is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat BTC amount of sample captured in plate.
Background: Betacellulin, also known as BTC is a protein that in humans is encoded by the BTC gene. This gene is mapped to 4q13.3. The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. It is the growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. This protein is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.
Background: Betacellulin, also known as BTC is a protein that in humans is encoded by the BTC gene. This gene is mapped to 4q13.3. The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. It is the growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. This protein is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
19,579 Da
NCBI Official Full Name
Betacellulin
NCBI Official Synonym Full Names
betacellulin
NCBI Official Symbol
Btc
NCBI Protein Information
probetacellulin
UniProt Protein Name
Betacellulin
Protein Family
UniProt Gene Name
Btc
UniProt Entry Name
Q9JJM4_RAT
NCBI Description
may have mitogenic activity; mouse homolog induces differentiation of pancreatic beta-cells and promotes regeneration of beta-cells [RGD, Feb 2006]
Research Articles on BTC
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Product Notes
The Rat BTC btc (Catalog #AAA177185) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177185 ELISA Kit recognizes Rat BTC. It is sometimes possible for the material contained within the vial of "BTC, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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