HPGD / 15-PGDH recombinant protein

Recombinant Mouse HPGD / 15-PGDH Protein (His tag)

Cat. Num. AAA2546932

• Gene Names: Hpgd; 15-PGDH; AV026552
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: HPGD / 15-PGDH; Recombinant Mouse HPGD / 15-PGDH Protein (His tag); 15-PGDH; AV026552; MGC14001; HPGD / 15-PGDH recombinant protein

• Host: E Coli
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 8.0, 20% glycerol
• Sequence Length: 269

• Application Notes: The recombinant mouse HPGD consisting of 279 amino acids and has a calculated molecular mass of 30.6 kDa. rmHPGD migrates as an approximately 30 kDa band in SDS-PAGE under reducing conditions as predicted.
• Predicted N Terminal: Met 1
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for HPGD / 15-PGDH recombinant protein

Background: Mouse 15-hydroxyprostaglandin dehydrogenase [NAD+], also known as Prostaglandin dehydrogenase 1, HPGD, and PGDH1, is a member of the short-chain dehydrogenases/reductases (SDR) family. Prostaglandins (PGs) play a key role in the onset of labor in many species and regulate uterine contractility and cervical dilatation. Therefore, the regulation of prostaglandin output by PG synthesizing and metabolizing enzymes in the human myometrium may determine uterine activity patterns in human labor both at preterm and at term. Prostaglandin dehydrogenase (PGDH) metabolizes prostaglandins (PGs) to render them inactive. HPGD is down-regulated by cortisol, dexamethasone and betamethasone and down-regulated in colon cancer. It is up-regulated by TGFB1. HPGD contributes to the regulation of events that are under the control of prostaglandin levels. HPGD catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. and inhibits in vivo proliferation of colon cancer cells. Defects in HPGD are the cause of primary hypertrophic osteoathropathy autosomal recessive (PHOAR) , cranioosteoarthropathy (COA), and isolated congenital nail clubbing.

Description: A DNA sequence encoding the mouse HPGD (Q8VCC1) (Met 1-Ser 269) was expressed, with a polyhistide tag at the C-terusmin.

NCBI and Uniprot Product Information

• NCBI GI #: 124486706
• NCBI GeneID: 15446
• NCBI Accession #: NP_032304.2
• NCBI GenBank Nucleotide #: NM_008278.2
• UniProt Accession #: Q8VCC1; Q61106
• Molecular Weight: 29,181 Da
• NCBI Official Full Name: 15-hydroxyprostaglandin dehydrogenase
• NCBI Official Synonym Full Names: hydroxyprostaglandin dehydrogenase 15 (NAD)
• NCBI Official Symbol: Hpgd
• NCBI Official Synonym Symbols: 15-PGDH; AV026552
• NCBI Protein Information: 15-hydroxyprostaglandin dehydrogenase [NAD(+)]
• UniProt Protein Name: 15-hydroxyprostaglandin dehydrogenase [NAD(+)]
• UniProt Gene Name: Hpgd
• UniProt Synonym Gene Names: Pgdh1; 15-PGDH

Uniprot Description

HPGD: Prostaglandin inactivation. Contributes to the regulation of events that are under the control of prostaglandin levels. Catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. Inhibits in vivo proliferation of colon cancer cells. Defects in HPGD are the cause of hypertrophic osteoarthropathy, primary, autosomal recessive, type 1 (PHOAR1). A disease characterized by digital clubbing, periostosis, acroosteolysis, painful joint enlargement, and variable features of pachydermia that include thickened facial skin and a thickened scalp. Other developmental anomalies include delayed closure of the cranial sutures and congenital heart disease. Defects in HPGD are the cause of cranioosteoarthropathy (COA). A form of osterarthropathy characterized by swelling of the joints, digital clubbing, hyperhidrosis, delayed closure of the fontanels, periostosis, and variable patent ductus arteriosus. Pachydermia is not a prominent feature. Defects in HPGD are a cause of isolated congenital nail clubbing (ICNC); also called clubbing of digits or hereditary acropachy. ICNC is a rare genodermatosis characterized by enlargement of the nail plate and terminal segments of the fingers and toes, resulting from proliferation of the connective tissues between the nail matrix and the distal phalanx. It is usually symmetrical and bilateral (in some cases unilateral). In nail clubbing usually the distal end of the nail matrix is relatively high compared to the proximal end, while the nail plate is complete but its dimensions and diameter more or less vary in comparison to normal. There may be different fingers and toes involved to varying degrees. Some fingers or toes are spared, but the thumbs are almost always involved. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.1.1.141; Oxidoreductase; Tumor suppressor

Chromosomal Location of Human Ortholog: 8|8 B2

Cellular Component: basolateral plasma membrane; cytoplasm; nucleoplasm

Molecular Function: 15-hydroxyprostaglandin dehydrogenase (NAD+) activity; catalytic activity; NAD binding; prostaglandin E receptor activity

Biological Process: female pregnancy; negative regulation of cell cycle; parturition; prostaglandin metabolic process; transforming growth factor beta receptor signaling pathway