Testing Data
Rabbit STAT5 (pY694) Antibody | anti-STAT5 (pY694) antibody
Rabbit anti Phospho- STAT5 (pTyr 694)
Reactivity
Human, Rat, Mouse, Bovine, Canine
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
Synonyms
STAT5 (pY694); Rabbit anti Phospho- STAT5 (pTyr 694); Rabbit anti-Phospho-STAT5 (pY694); Signal transducer and activator of transcription 5 (STAT5); anti-STAT5 (pY694) antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse, Bovine, Canine
Specificity
This antibody recognizes STAT5 (pY694) with a phosphorylated sites of Tyr694. It does not cross-react with non-phosphospecific peptide.
Purity/Purification
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
Concentration
Size: 100 ug/200 ul (varies by lot)
Applicable Applications for anti-STAT5 (pY694) antibody
ELISA, Western Blot, Immunohistochemistry
Application Notes
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 1:100
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 1:100
Antigen Preparation
A synthetic peptide surrounding to the epitope -DGYVK-- with phosphorylation sites at Tyr694 of STAT5 protein from human, mouse and rat origins.
Positive Control
GH stimulated mouse liver
Cellular Location
Nuclear
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Testing Data
Testing Data
Related Product Information for anti-STAT5 (pY694) antibody
STAT proteins (Signal transducer and activator of transcription) belong to a family of cytoplasmic transcription factors that can be phosphorylated by a lignad binding to its cell surface receptor. STAT5 is tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Serine phosphorylation is also required for maximal transcriptional activity. NCoA-1/SRC-1 acts as a co-activator for both the a- and b-isoforms of STAT5.
References
Lin, J.X., et al. Cloning of human Stat5B. Reconstitution of interleukin-2-induced Stat5A and Stat5B DNA binding activity in COS-7 cells. J. Biol Chem. 271: 10738, 2006
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
92 kDa
NCBI Official Full Name
STAT5
NCBI Official Symbol
stat5a
NCBI Protein Information
STAT5
Research Articles on STAT5 (pY694)
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Product Notes
The STAT5 (pY694) (Catalog #AAA462116) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Phospho- STAT5 (pTyr 694) reacts with Human, Rat, Mouse, Bovine, Canine and may cross-react with other species as described in the data sheet. AAA Biotech's STAT5 (pY694) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunohistochemistry. Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml Immunohistochemistry: 1:100. Researchers should empirically determine the suitability of the STAT5 (pY694) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STAT5 (pY694), Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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