Mouse Stat5 (Tyr-694) Monoclonal Antibody | anti-STAT5A antibody

Stat5 (Tyr-694), phospho-specific

Cat. Num. AAA470030

• Gene Names: STAT5A; MGF; STAT5
• Reactivity: Human, Rat, Mouse
• Applications: ELISA, Western Blot
• Purity: Protein A Purified
• Synonyms: Stat5 (Tyr-694); Monoclonal Antibody; phospho-specific; Signal transducer and activator of transcription 5A; Stat5A; anti-STAT5A antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: The antibody detects a 92 kDa* band corresponding to Stat5A on SDS-PAGE immunoblots of human A431 cells treated with EGF, but does not detect this band in control cells.
• Purity/Purification: Protein A Purified
• Form/Format: Mouse monoclonal antibody purified with protein A chromatography is supplied in 100ml phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
• Sequence Length: 794

• Applicable Applications for anti-STAT5A antibody: ELISA, Western Blot
• Application Notes: ELISA: 1:2000; Western Blot: 1:1000
• Immunogen: Clone (M148) was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 694 of human Stat5A. This peptide sequence is identical to tyrosine 699 in human Stat5B, and has high homology to the conserved tyrosine site in rat and mouse Stat5A/B.
• Preparation and Storage: Store at -20 degree C. Do not aliquot. Stable for 1 year.

Testing Data

Related Product Information for anti-STAT5A antibody

The stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr-694 is required for Stat5A activation. Stat5 has been found to be constitutively active in some leukemic cell types.Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility. Both Stat5A (Tyr-694) and Stat5B (Tyr-699) are independently regulated and activated in various cell types. For instance, both isoforms are activated in response to IFN-alpha in B cells, but only Stat5alpha is phosphorylated in response to IFN-alpha in HeLa cells.

References

• Gouilleux, F. et al. (1994) EMBO J. 13:4361.
• Wakao, H. et al. (1994) EMBO J. 13:2182.
• Wakao, H. et al. (1994) EMBO J. 13:2182.
• Darnell, J.E. (1997) Science 277:1630.

NCBI and Uniprot Product Information

• NCBI GI #: 21618342
• NCBI GeneID: 6776
• NCBI Accession #: NP_003143.2
• NCBI GenBank Nucleotide #: NM_003152.3
• UniProt Accession #: P42229; Q1KLZ6; Q59GY7; Q8WWS9
• Molecular Weight: 90516 Da
• NCBI Official Full Name: signal transducer and activator of transcription 5A
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 5A
• NCBI Official Symbol: STAT5A
• NCBI Official Synonym Symbols: MGF; STAT5
• NCBI Protein Information: signal transducer and activator of transcription 5A; OTTHUMP00000198376
• UniProt Protein Name: Signal transducer and activator of transcription 5A
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: STAT5A
• UniProt Synonym Gene Names: STAT5
• UniProt Entry Name: STA5A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq]

Uniprot Description

Function: Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.

Subunit structure: Forms a homodimer or a heterodimer with a related family member. Binds NR3C1

By similarity. Interacts with NCOA1 and SOCS7. Ref.4 Ref.5

Subcellular location: Cytoplasm. Nucleus. Note: Translocated into the nucleus in response to phosphorylation.

Post-translational modification: Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity

By similarity. Ref.6 Ref.7 Ref.8 Ref.9 Ref.10

Sequence similarities: Belongs to the transcription factor STAT family.Contains 1 SH2 domain.