Testing Data
Rabbit STAT1 (pY701) Antibody | anti-STAT1 (pY701) antibody
Rabbit anti Phospho- STAT1 (pTyr 701)
Gene Names
stat1; XStat1
Reactivity
Human, Rat, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
Synonyms
STAT1 (pY701); Rabbit anti Phospho- STAT1 (pTyr 701); Rabbit anti-Phospho-STAT1 (pY701); Signal transducer and activator of transcription 1 (STAT1); anti-STAT1 (pY701) antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Specificity
This antibody recognizes STAT1 (pY701) with a phosphorylated sites of Tyr701. It does not cross-react with non-phosphospecific peptide.
Purity/Purification
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
Concentration
Size: 100 ug/200 ul (varies by lot)
Applicable Applications for anti-STAT1 (pY701) antibody
ELISA, Western Blot, Immunohistochemistry
Application Notes
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 1:50
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 1:50
Antigen Preparation
A synthetic peptide surrounding to the epitope -TGYIK-- with phosphorylation sites at Tyr701 of STAT1 protein from human, mouse and rat origins.
Positive Control
GH stimulated mouse liver
Cellular Location
Nuclear
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Testing Data
Testing Data
Related Product Information for anti-STAT1 (pY701) antibody
STAT proteins (Signal transducer and activator of transcription) belong to a family of cytoplasmic transcription factors that can be phosphorylated by a lignad binding to its cell surface receptor. The phosphorylation of STAT1 at Tyr701 induces STAT1 dimerization, nuclear translocation and DNA binding. STAT 1 is activated by a number of different ligands, including IFN, EGF, PDGF and IL6. Tyrosine 701 phosphorylation impairment results in loss of STAT 1 functions.
References
Tong R Wu, et al. SHP-2 Is a Dual-specificity Phosphatase Involved in Stat1 Dephosphorylation at Both Tyrosine and Serine Residues in Nuclei, J. Biol. Chem., Vol. 277, Issue 49, 47572-47580, December 6, 2002
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
91 kDa
NCBI Official Full Name
signal transducer and activator of transcription 1, 91kDa
NCBI Official Synonym Full Names
signal transducer and activator of transcription 1, 91kDa
NCBI Official Symbol
stat1
NCBI Official Synonym Symbols
XStat1
NCBI Protein Information
signal transducer and activator of transcription 1, 91kDa
Similar Products
Product Notes
The STAT1 (pY701) (Catalog #AAA462111) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Phospho- STAT1 (pTyr 701) reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's STAT1 (pY701) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunohistochemistry. Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml Immunohistochemistry: 1:50. Researchers should empirically determine the suitability of the STAT1 (pY701) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STAT1 (pY701), Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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