Rabbit STAT6 (pY641) Antibody | anti-STAT6 (pY641) antibody

Rabbit anti Phospho- STAT6 (pTyr 641)

Cat. Num. AAA462172

• Gene Names: STAT6; STAT6B; STAT6C; D12S1644; IL-4-STAT
• Reactivity: Human, Rat, Mouse,
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
• Synonyms: STAT6 (pY641); Rabbit anti Phospho- STAT6 (pTyr 641); Rabbit anti-Phospho-STAT6 (pY641); Signal transducer and activator of transcription 6 (STAT6); IL-4-STAT; anti-STAT6 (pY641) antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse,
• Specificity: This antibody recognizes STAT6 (pY641) with a phosphorylated sites of Tyr641. It does not cross-react with non-phosphospecific peptide.
• Purity/Purification: The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
• Form/Format: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
• Concentration: Size: 100 ug/200 ul (varies by lot)

• Applicable Applications for anti-STAT6 (pY641) antibody: ELISA, Western Blot, Immunohistochemistry
• Application Notes: Western Blot: 0.1-1 ug/ml; ELISA: 0.01-0.1 ug/ml; Immunoprecipitation: 2-5 ug/ml; Immunohistochemistry: 1:100 ug/ml
• Antigen Preparation: A synthetic peptide surrounding to the epitope -RGYVP-- with phosphorylation sites at Tyr641 of STAT6 protein from human, mouse and rat origins.
• Positive Control: HepG2
• Cellular Location: Nuclear
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Testing Data

Related Product Information for anti-STAT6 (pY641) antibody

STAT proteins (Signal transducer and activator of transcription) belong to a family of cytoplasmic transcription factors that can be phosphorylated by a ligand binding to its cell surface receptor. Activation by phosphorylation of STAT6 at tyrosine 641 upon the IL-4 (possibly IL-13) ligand binding to its receptor induces dimerization, nuclear translocation and DNA binding. STAT6 is widely expressed in human tissues. It exhibits elevated expression in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung, and placenta. STAT6 mediates the IL-4 signal and is essential for the proper development of adaptive immunity.

References

Zhiguang Li et al (2008). Endogenous Interleukin-4 Promotes Tumor Development by Increasing Tumor Cell Resistance to Apoptosis. Cancer Research 68, 8687-94.

NCBI and Uniprot Product Information

• NCBI GI #: 23397678
• NCBI GeneID: 6778
• NCBI Accession #: NP_003144.3
• NCBI GenBank Nucleotide #: NM_003153.4
• UniProt Accession #: P42226; Q5FBW5; Q71UP4; A8K316; Q56TK0; Q5FBW6; Q6LCD8; Q9BQD2
• Molecular Weight: 95 kDa
• NCBI Official Full Name: signal transducer and activator of transcription 6 isoform 1
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 6, interleukin-4 induced
• NCBI Official Symbol: STAT6
• NCBI Official Synonym Symbols: STAT6B; STAT6C; D12S1644; IL-4-STAT
• NCBI Protein Information: signal transducer and activator of transcription 6; STAT, interleukin4-induced; transcription factor IL-4 STAT
• UniProt Protein Name: Signal transducer and activator of transcription 6
• UniProt Gene Name: STAT6
• UniProt Entry Name: STAT6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Alternative splicing results in multiple transcript variants.

Uniprot Description

Function: Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.

Subunit structure: Forms a homodimer or a heterodimer with a related family member

By similarity. Interacts with NCOA1 via its C-terminal LXXLL motif. Ref.7

Subcellular location: Cytoplasm. Nucleus. Note: Translocated into the nucleus in response to phosphorylation.

Post-translational modification: Tyrosine phosphorylated following stimulation by IL-4 and IL-3

Sequence similarities: Belongs to the transcription factor STAT family.Contains 1 SH2 domain.