Rabbit STAT4 (pY693) Antibody | anti-STAT4 (pY693) antibody

Rabbit anti Phospho- STAT4 (pTyr 693)

Cat. Num. AAA462115

• Gene Names: STAT4; SLEB11
• Reactivity: Human, Rat, Mouse, Canine
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
• Synonyms: STAT4 (pY693); Rabbit anti Phospho- STAT4 (pTyr 693); Rabbit anti-Phospho-STAT4 (pY693); Signal transducer and activator of transcription 4 (STAT4); anti-STAT4 (pY693) antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse, Canine
• Specificity: This antibody recognizes STAT4 (pY693) with a phosphorylated sites of Tyr693. It does not cross-react with non-phosphospecific peptide.
• Purity/Purification: The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
• Form/Format: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
• Concentration: Size: 100 ug/200 ul (varies by lot)

• Applicable Applications for anti-STAT4 (pY693) antibody: ELISA, Western Blot, Immunohistochemistry
• Application Notes: Western Blot: 0.1-1 ug/ml; ELISA: 0.01-0.1 ug/ml; Immunoprecipitation: 2-5 ug/ml; Immunohistochemistry: 1:50 ug/ml
• Antigen Preparation: A synthetic peptide surrounding to the epitope -KGYVP-- with phosphorylation sites at Tyr693 of STAT4 protein from human, mouse, rat, chicken dog and bovine origins.
• Positive Control: Mouse kidney
• Cellular Location: Nuclear
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Testing Data

Related Product Information for anti-STAT4 (pY693) antibody

STAT proteins (Signal transducer and activator of transcription) belong to a family of cytoplasmic transcription factors that can be phosphorylated by a lignad binding to its cell surface receptor. Activation of STAT4 induces dimerization, nuclear translocation and DNA binding. STAT4 protein expression is restricted to the thymus, spleen and testis. Both tyrosine and serine residues on STAT4 become phosphorylated in response to IL-12 or IFN-alpha although serine phosphorylation is not required for DNA binding. STAT4 is activated in T-cells in response to the cytokine interleukin-12 (IL-12), and in NK cells by IL-2. STAT4-deficient mice display impaired IL-12 development of Th1 cells and enhanced development of Th2 cells.

References

Takuya Miyagi, et al. High basal STAT4 balanced by STAT1 induction to control type 1 interferon effects in natural killer cells. J Exp Med. 2007 October 1; 204 (10): 2383-2396.

NCBI and Uniprot Product Information

• NCBI GI #: 4507255
• NCBI GeneID: 6775
• NCBI Accession #: NP_003142.1
• NCBI GenBank Nucleotide #: NM_003151.2
• UniProt Accession #: Q14765; Q96NZ6; Q53RU2; Q53S87; Q59GR6
• Molecular Weight: 90 kDa
• NCBI Official Full Name: signal transducer and activator of transcription 4
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 4
• NCBI Official Symbol: STAT4
• NCBI Official Synonym Symbols: SLEB11
• NCBI Protein Information: signal transducer and activator of transcription 4; OTTHUMP00000163553; OTTHUMP00000205847
• UniProt Protein Name: Signal transducer and activator of transcription 4
• UniProt Gene Name: STAT4
• UniProt Entry Name: STAT4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. [provided by RefSeq]

Uniprot Description

Function: Carries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling.

Subunit structure: Forms a homodimer or a heterodimer with a related family member. Interacts with ARL2BP

By similarity. The SH2 domain interacts, in vitro, with IL12RB2 via a short cytoplasmic domain. Ref.4 Ref.5

Subcellular location: Cytoplasm. Nucleus. Note: Translocated into the nucleus in response to phosphorylation.

Post-translational modification: Tyrosine phosphorylated. Serine phosphorylation is also required for maximal transcriptional activity

Involvement in disease: Genetic variations in STAT4 are associated with susceptibility to systemic lupus erythematosus type 11 (SLEB11) [

MIM:612253]. Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with a complex genetic basis. SLE is an inflammatory, and often febrile multisystemic disorder of connective tissue characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Ref.7Genetic variations in STAT4 are a cause of susceptibility to rheumatoid arthritis (RA) [

MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Ref.7

Sequence similarities: Belongs to the transcription factor STAT family.Contains 1 SH2 domain.