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Typical Testing Data/Standard Curve (for reference only)

Rabbit TNF-beta ELISA Kit | TNF-beta elisa kit

Rabbit TNF-beta (Tumor Necrosis Factor Beta) ELISA Kit

Gene Names
Lta; LT; Ltx; Tnfb; LT[a]; LT-[a]; TNFSF1; hlb382; LTalpha; Tnfsf1b; LT-alpha; TNF-beta
Reactivity
Rabbit
Synonyms
TNF-beta; Rabbit TNF-beta (Tumor Necrosis Factor Beta) ELISA Kit; TNF-beta elisa kit
Ordering
For Research Use Only!
Reactivity
Rabbit
Specificity
This kit recognizes Rabbit TNF-beta in samples. No significant cross-reactivity or interference between Rabbit TNF-beta and analogues was observed.
Sequence Length
202
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
15.625-1000pg/mL
Sensitivity
9.375pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rabbit TNF-beta were tested 20 times on one plate, respectively.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rabbit TNF-beta were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TNF-beta elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rabbit TNF-beta concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit TNF-beta. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit TNF-beta and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit TNF-beta, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rabbit TNF-beta. You can calculate the concentration of Rabbit TNF-beta in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
21,999 Da
NCBI Official Full Name
TNF-beta
NCBI Official Synonym Full Names
lymphotoxin A
NCBI Official Symbol
Lta
NCBI Official Synonym Symbols
LT; Ltx; Tnfb; LT[a]; LT-[a]; TNFSF1; hlb382; LTalpha; Tnfsf1b; LT-alpha; TNF-beta
NCBI Protein Information
lymphotoxin-alpha; TNF beta; lymphotoxin alpha; tumor necrosis factor ligand superfamily member 1
UniProt Protein Name
Lymphotoxin-alpha
UniProt Gene Name
Lta
UniProt Synonym Gene Names
Tnfb; Tnfsf1; LT-alpha
UniProt Entry Name
TNFB_MOUSE

Uniprot Description

LTA: Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo. Genetic variations in LTA are a cause of susceptibility psoriatic arthritis (PSORAS). PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). Belongs to the tumor necrosis factor family.

Protein type: Secreted, signal peptide; Apoptosis; Cytokine; Secreted

Cellular Component: extracellular space; membrane; extracellular region

Molecular Function: protein binding; cytokine activity; tumor necrosis factor receptor binding

Biological Process: positive regulation of humoral immune response mediated by circulating immunoglobulin; transformed cell apoptosis; positive regulation of apoptosis; lymph node development; humoral immune response; cell proliferation; positive regulation of interferon-gamma production; defense response to Gram-positive bacterium; negative regulation of fibroblast proliferation; positive regulation of cell proliferation; positive regulation of chronic inflammatory response to antigenic stimulus; immune response; inflammatory response; cellular process

Research Articles on TNF-beta

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Product Notes

The Rabbit TNF-beta lta (Catalog #AAA2504802) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2504802 ELISA Kit recognizes Rabbit TNF-beta. It is sometimes possible for the material contained within the vial of "TNF-beta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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