Bioactivity
(Connective Tissue Growth Factor (CTGF), also known as CCN2 is a matricellular protein of the CCN family of extracellular matrix-associated heparin-binding proteins. CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers. As CTGF has the function of cell adhesion, we measure the activity of recombinant human CTGF by the ability of the immobilized protein to support the adhesion of Balb/3T3 mouse embryonic fibroblast cell. When 5 x 104 cells/well are added to different concentrations of recombinat human CTGF coated plates, cells will adhere after 2 hour incubation at 37 degree C. The adhesion of Balb/3T3 after 2 hour incubation at 37 degree C observed by inverted microscope was shown in Figure 1. Cell adherent was in a dose dependent manner, the result was shown in Figure 2, the EC50 was 0.6 ug/ml.)
Connective Tissue Growth Factor (CTGF) Active Protein | CTGF active protein
Active Connective Tissue Growth Factor (CTGF)
Gene Names
CTGF; CCN2; NOV2; HCS24; IGFBP8
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Synonyms
Connective Tissue Growth Factor (CTGF); N/A; Active Connective Tissue Growth Factor (CTGF); Connective Tissue Growth Factor; CCN2; HCS24; IGFBP8; NOV2; Insulin-Like Growth Factor-Binding Protein 8; Hypertrophic Chondrocyte-Specific Protein 24; CCN Family Member 24; CTGF active protein
Host
E Coli
Purity/Purification
Greater than 80% by SDS-PAGE
Form/Format
Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Sequence
Gln27~Ala349
Applicable Applications for CTGF active protein
Activity Assay, Cell Culture
Species
Human
Expression System
Prokaryotic expression
Tag
N-terminal His and GST Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Isoelectric Point
7.9
Research Area
Cytokine
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Bioactivity
(Connective Tissue Growth Factor (CTGF), also known as CCN2 is a matricellular protein of the CCN family of extracellular matrix-associated heparin-binding proteins. CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers. As CTGF has the function of cell adhesion, we measure the activity of recombinant human CTGF by the ability of the immobilized protein to support the adhesion of Balb/3T3 mouse embryonic fibroblast cell. When 5 x 104 cells/well are added to different concentrations of recombinat human CTGF coated plates, cells will adhere after 2 hour incubation at 37 degree C. The adhesion of Balb/3T3 after 2 hour incubation at 37 degree C observed by inverted microscope was shown in Figure 1. Cell adherent was in a dose dependent manner, the result was shown in Figure 2, the EC50 was 0.6 ug/ml.)
Bioactivity
(Connective Tissue Growth Factor (CTGF), also known as CCN2 is a matricellular protein of the CCN family of extracellular matrix-associated heparin-binding proteins. CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers. As CTGF has the function of cell adhesion, we measure the activity of recombinant human CTGF by the ability of the immobilized protein to support the adhesion of Balb/3T3 mouse embryonic fibroblast cell. When 5 x 104 cells/well are added to different concentrations of recombinat human CTGF coated plates, cells will adhere after 2 hour incubation at 37 degree C. The adhesion of Balb/3T3 after 2 hour incubation at 37 degree C observed by inverted microscope was shown in Figure 1. Cell adherent was in a dose dependent manner, the result was shown in Figure 2, the EC50 was 0.6 ug/ml.)
SDS-PAGE
(Figure. SDS-PAGE)
SDS-PAGE
(Figure. SDS-PAGE)
Product Categories/Family for CTGF active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,091 Da
NCBI Official Full Name
connective tissue growth factor
NCBI Official Synonym Full Names
connective tissue growth factor
NCBI Official Symbol
CTGF
NCBI Official Synonym Symbols
CCN2; NOV2; HCS24; IGFBP8
NCBI Protein Information
connective tissue growth factor; IBP-8; IGFBP-8; CCN family member 2; IGF-binding protein 8; hypertrophic chondrocyte-specific protein 24; insulin-like growth factor-binding protein 8
UniProt Protein Name
Connective tissue growth factor
UniProt Gene Name
CTGF
UniProt Synonym Gene Names
CCN2; HCS24; IGFBP8; IBP-8; IGF-binding protein 8; IGFBP-8
UniProt Entry Name
CTGF_HUMAN
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Product Notes
The CTGF ctgf (Catalog #AAA161649) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Connective Tissue Growth Factor (CTGF) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the CTGF ctgf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Gln27~Ala3 49. It is sometimes possible for the material contained within the vial of "Connective Tissue Growth Factor (CTGF), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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