Typical Testing Data/Standard Curve (for reference only)
Human Tumor necrosis factor beta, TNF-beta ELISA Kit | TNF-beta elisa kit
Human Tumor necrosis factor beta, TNF-beta ELISA Kit
Gene Names
Lta; LT; Ltx; Tnfb; LT[a]; LT-[a]; TNFSF1; Tnlg1e; hlb382; LTalpha; Tnfsf1b; LT-alpha; TNF-beta
Reactivity
Human
Synonyms
Tumor necrosis factor beta; TNF-beta; Human Tumor necrosis factor beta; TNF-beta ELISA Kit; TNF-beta elisa kit
Reactivity
Human
Sequence Length
202
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
2ng/L - 600ng/L
Sensitivity
1.05ng/L
Intra-Assay
CV<8%
Inter-Assay
CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TNF-beta elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Tumor Necrosis Factor Beta (also known as TNF-B) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human TNF-B antibody. TNF-B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human TNF-B Antibody is added and binds to TNF-B in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TNF-B antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human TNF-B. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human TNF-B antibody. TNF-B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human TNF-B Antibody is added and binds to TNF-B in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TNF-B antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human TNF-B. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
21,999 Da
NCBI Official Full Name
TNF-beta
NCBI Official Synonym Full Names
lymphotoxin A
NCBI Official Symbol
Lta
NCBI Official Synonym Symbols
LT; Ltx; Tnfb; LT[a]; LT-[a]; TNFSF1; Tnlg1e; hlb382; LTalpha; Tnfsf1b; LT-alpha; TNF-beta
NCBI Protein Information
lymphotoxin-alpha
UniProt Protein Name
Lymphotoxin-alpha
UniProt Gene Name
Lta
UniProt Synonym Gene Names
Tnfb; Tnfsf1; LT-alpha
Uniprot Description
Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo.
Research Articles on TNF-beta
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Product Notes
The Human TNF-beta lta (Catalog #AAA165237) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA165237 ELISA Kit recognizes Human TNF-beta. It is sometimes possible for the material contained within the vial of "Tumor necrosis factor beta, TNF-beta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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