APOM recombinant protein

Recombinant Human APOM Protein

Cat. Num. AAA286217

• Gene Names: APOM; G3a; NG20; apo-M; HSPC336
• Purity: > 90 % as determined by SDS-PAGE.
• Synonyms: APOM; Recombinant Human APOM Protein; G3a; HSPC336; NG20; apo-M; APOM recombinant protein

• Host: E. coli AA 1-188 (O95445).
• Purity/Purification: > 90 % as determined by SDS-PAGE.
• Form/Format: 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, 50% glycerol.
• Concentration: 2.1 mg/mL (varies by lot)
• Sequence: MFHQIWAALLYFYGIILNSIYQCPEHSQLTTLGVDGKEFPEVHLGQWYFIAGAAPTKEELATFDPVDNIVFNMAAGSAPMQLHLRATIRMKDGLCVPRKWIYHLTEGSTDLRTEGRPDMKTELFSSSCPGGIMLNETGQGYQRFLLYNRSPHPPEKCVEEFKSLTSCLDSKAFLLTPRNQEACELSNN

• Species: Human
• Source: Human
• Protein Residues: with N-terminal 10×His-GST-tagged.
• Endotoxin Level: Please contact us for more information.
• Usage: APOM Protein - Centrifuge the standard vial at 6000-10000rpm for 30s.
• Preparation and Storage: Store it under sterile conditions at -20°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. The recombinant protein is stable for up to 6-12 months from date of receipt at -20°C to -80°C. **Avoid repeated freeze-thaw cycles.**

SDS-PAGE

Related Product Information for APOM recombinant protein

Apolipoprotein M is an apolipoprotein and member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. Two transcript variants encoding two different isoforms have been found for this gene, but only one of them has been fully characterized. The deduced 188-amino acid protein has a calculated molecular mass of about 21 kD. It contains a potential N-glycosylation site, 2 possible disulfide bridges, and a hydrophobic alpha-helical signal peptide that is retained in the mature protein. Sequence analysis revealed 79% identity between the human and mouse proteins.

NCBI and Uniprot Product Information

• NCBI GI #: 371873523
• NCBI GeneID: 55937
• NCBI Accession #: NP_001243098.1
• NCBI GenBank Nucleotide #: NM_001256169.1
• UniProt Accession #: O95445; Q5SRP4; Q9P046; Q9UMP6; B0UX98
• Molecular Weight: Predicted MW: 49.8 kDa; Observed MW: 50 kDa
• NCBI Official Full Name: apolipoprotein M isoform 2
• NCBI Official Synonym Full Names: apolipoprotein M
• NCBI Official Symbol: APOM
• NCBI Official Synonym Symbols: G3a; NG20; apo-M; HSPC336
• NCBI Protein Information: apolipoprotein M
• UniProt Protein Name: Apolipoprotein M
• Protein Family: Apomucin
• UniProt Gene Name: APOM
• UniProt Synonym Gene Names: G3A; NG20; Apo-M; ApoM
• UniProt Entry Name: APOM_HUMAN

NCBI Description

The protein encoded by this gene is an apolipoprotein and member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. Alternate splicing results in both coding and non-coding variants of this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

APOM: Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid. Plasma protein. Expressed in liver and kidney. Belongs to the calycin superfamily. Lipocalin family. Highly divergent.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6p21.33

Cellular Component: extracellular region; integral to plasma membrane

Molecular Function: antioxidant activity; lipid transporter activity; phospholipid binding

Biological Process: cholesterol efflux; cholesterol homeostasis; retinoid metabolic process; reverse cholesterol transport