Typical Testing Data/Standard Curve (for reference only)
Chicken Tumor Necrosis Factor beta ELISA Kit | TNFbeta elisa kit
Chicken Tumor Necrosis Factor beta ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: TNF-beta ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TNF-beta antibody and an TNF-beta-HRP conjugate. The assay sample and buffer are incubated together with TNF-beta-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TNF-beta concentration since TNF-beta from samples and TNF-beta-HRP conjugate compete for the anti-TNF-beta antibody binding site. Since the number of sites is limited, as more sites are occupied by TNF-beta from the sample, fewer sites are left to bind TNF-beta-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TNF-beta concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
LTA: Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo. Genetic variations in LTA are a cause of susceptibility psoriatic arthritis (PSORAS). PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). Belongs to the tumor necrosis factor family.
Protein type: Secreted; Cytokine; Apoptosis; Secreted, signal peptide
Cellular Component: extracellular space; membrane; extracellular region
Molecular Function: protein binding; cytokine activity; tumor necrosis factor receptor binding
Biological Process: positive regulation of humoral immune response mediated by circulating immunoglobulin; transformed cell apoptosis; positive regulation of apoptosis; lymph node development; humoral immune response; cell proliferation; positive regulation of interferon-gamma production; defense response to Gram-positive bacterium; negative regulation of fibroblast proliferation; positive regulation of cell proliferation; immune response; positive regulation of chronic inflammatory response to antigenic stimulus; inflammatory response; cellular process
Research Articles on TNFbeta
Similar Products
Product Notes
The Chicken TNFbeta lta (Catalog #AAA736116) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA736116 ELISA Kit recognizes Chicken TNFbeta. It is sometimes possible for the material contained within the vial of "Tumor Necrosis Factor beta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
