Typical Testing Data/Standard Curve (for reference only)
Mouse ERalpha (Estrogen Receptor Alpha) CLIA Kit | ERa clia kit
Mouse ERalpha (Estrogen Receptor Alpha) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to ERalpha. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ERalpha and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ERalpha, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of ERalpha. You can calculate the concentration of ERalpha in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
ER-alpha: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described.
Protein type: DNA-binding; Nuclear receptor
Chromosomal Location of Human Ortholog: 6q25.1
Cellular Component: cytoplasm; cytosol; Golgi apparatus; integral to membrane; membrane; mitochondrion; nuclear chromatin; nucleoplasm; nucleus; perikaryon; perinuclear region of cytoplasm; plasma membrane; protein complex; T-tubule; terminal button
Molecular Function: ATPase binding; beta-catenin binding; chromatin binding; enzyme binding; estrogen receptor activity; estrogen response element binding; hormone binding; identical protein binding; nitric-oxide synthase regulator activity; protein binding; protein complex binding; steroid binding; steroid hormone receptor activity; transcription factor activity; transcription factor binding; type 1 metabotropic glutamate receptor binding; zinc ion binding
Biological Process: androgen metabolic process; antral ovarian follicle growth; chromatin remodeling; decidualization; elevation of cytosolic calcium ion concentration; estrogen receptor signaling pathway; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); gene expression; negative regulation of glucose import; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of smooth muscle cell proliferation; negative regulation of transcription factor activity; osteoblast development; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of epithelial cell proliferation; positive regulation of fibroblast proliferation; positive regulation of nitric oxide biosynthetic process; positive regulation of nitric-oxide synthase activity; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of neuron apoptosis; regulation of transcription, DNA-dependent; response to estradiol stimulus; response to estrogen stimulus; Sertoli cell development; Sertoli cell proliferation; signal transduction; steroid hormone mediated signaling; steroid hormone receptor signaling pathway; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; uterus development; vagina development
Disease: Breast Cancer; Estrogen Resistance; Migraine With Or Without Aura, Susceptibility To, 1; Myocardial Infarction, Susceptibility To
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Product Notes
The Mouse ERa esr1 (Catalog #AAA2531550) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2531550 CLIA Kit recognizes Mouse ERa. It is sometimes possible for the material contained within the vial of "ERalpha (Estrogen Receptor Alpha), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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