anti-Human, Mouse Angiopoietin-2 Monoclonal Antibody | anti-ANG-2 antibody

Mouse Anti-Angiopoietin-2 (Ang-2, Ang2, Angpt2, Agpt2)

Cat. Num. AAA6120615

• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: Purified by Protein A affinity chromatography.
• Synonyms: Angiopoietin-2; Monoclonal Antibody; Mouse Anti-Angiopoietin-2 (Ang-2; Ang2; Angpt2; Agpt2); anti-ANG-2 antibody

• Host: Host: Mouse; Source: Human
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG2b,l
• Clone Number: Angy-2-1
• Specificity: Recognizes human Angiopoietin-2. Species Crossreactivity: mouse. Does not detect human Angiopoietin-1.
• Purity/Purification: Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide, 10% glycerol.

• Applicable Applications for anti-ANG-2 antibody: ELISA (EIA)
• Application Notes: Fuctional Assay: Inhibits the binding of mouse angiopoietin-2 to mouse Tie-2. ND50=50-60ng/ml (for 10ng/ml of mouse Angiopoietin-2) Inhibits the binding of human angiopoietin-2 to human Tie-2. ND50=8-12ng/ml (for 10ng/ml of human Angiopoietin-2) ND50: 50% neutralizing dose of antibody for a given concentration of ligand (here Angiopoietin-2). Optimal dilutions to be determined by the researcher.
• Immunogen: Recombinant protein corresponding to human Angiopoietin-2 expressed in CHO cells.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Structure

(Structure of the recombinant antibody anti-Angiopoietin-2, mAb (rec.) (blocking) The single chain variable human fragment (ScFv) selected by antibody phage display technology and specific to the antigen of interest is fused to a mouse IgG2b Fc region.)

ELISA (EIA)

(Binding of human Angiopoietin-2 to Tie-2 (human): Fc is inhibited by the antibody anti-Angiopoietin-2, mAb (rec.) (blocking).|Methods: Tie-2 (h): Fc was coated on an ELISA plate at 1ug/ml. anti-Angiopoietin-2, mAb (rec.) (blocking) or an unrelated mAb (recombinant) (Control) were added (starting at 40µg/ml with a twofold serial dilution) together with 20ng/ul of Angiopoietin-2 (human). After incubation for 1h at RT, the binding was detected using an anti-DYKDDDDK antibody (HRP).)

Related Product Information for anti-ANG-2 antibody

Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) are closely related secreted ligands which bind with similar affinity to Tie-2. Tie-2 and angiopoietins have been shown to play critical roles in embryogenic angiogenesis and in maintaining the integrity of the adult vasculature. Ang-1 activates Tie-2 signaling on endothelial cells to promote chemotaxis, cell survival, cell sprouting, vessel growth and stabilization. Ang-2 has been identified as a secreted protein ligand of Tie-2 and has alternatively been reported to be an antagonist for Ang-1 induced Tie-2 signaling as well as an agonist for Tie-2 signaling, depending on the cell context. Angiopoietin-2 (human), mAb (rec.) (blocking) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein. Angiopoietin-2, is composed of human variable regions (VH and VL) (lamda-chain) of immunoglobulin fused to the mouse lgG2b Fc domain.