Sheep Tyrosine Hydroxylase Polyclonal Antibody | anti-TH antibody

Anti-Tyrosine Hydroxylase

Cat. Num. AAA502131

• Gene Names: Th; The
• Reactivity: All mammalian
• Applications: Western Blot, Immunofluorescence, Immunohistochemistry, Dot Blot
• Purity: Affinity Purified (Prepared from sheep serum by affinity purification using a column to which immunogen was coupled. The antibody is predominantly of the IgG1 subclass.)
• Synonyms: Tyrosine Hydroxylase; Polyclonal Antibody; Anti-Tyrosine Hydroxylase; anti-TH antibody

• Host: Sheep
• Reactivity: All mammalian
• Clonality: Polyclonal
• Specificity: Specific for the ~60k tyrosine Hydroxylase protein in Western Blot of rat brain lysate and PC-12 cells stimulated by Okadaic acid. The antibody cross-reacts with all mammalian and at least some non-mammalian forms of the enzyme in WB and IF/IHC.
• Purity/Purification: Affinity Purified (Prepared from sheep serum by affinity purification using a column to which immunogen was coupled. The antibody is predominantly of the IgG1 subclass.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots
• Sequence Length: 498

• Applicable Applications for anti-TH antibody: Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Dot Blots (DB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000; IHC (frozen sections; unpublished observations): 1:1000; IF (frozen sections; unpublished observations): 1:1000; DB: 1:1000
• Antigen: Native rat tyrosine hydroxylase, purified from pheochromocytoma.
• Immunogen Information: SDS-denatured, native rat tyrosine hydroxylase purified from pheochromocytoma
• Immunogen Species: Rat
• Species Reactivity Note: The antibody recognizes all mammalian and at least some non-mammalian forms of the enzyme in Western blots and in IHC/IF.
• Biological Significance: Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al.,
• Preparation and Storage: For long term storage -80 degree C is recommended, but shorter term storage at -20 degree C is also acceptable as aliquotes may be taken without freeze/thwaing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20 degree C.

Testing Data

Related Product Information for anti-TH antibody

Affinity purified sheep polyclonal antibody

References

• Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock JW (2001) Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567.
• Salvatore MF, Waymire JC, Haycock JW (2001) Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ. J Neurochem 79:349-360.
• Witkovsky P, Gabriel R, Haycock JW, Meller E (2000) Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina. J Chem Neuroanat 19:105-116.
• Zhu MY, Klimek V, Haycock JW, Ordway GA (2000) Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44
• Zhu MY, Klimek V, Dilley GE, Haycock JW, Stockmeier C, Overholser JC, Meltzer HY, Ordway GA (1999) Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression. Biol Psychiatry 46:1275-1286.

NCBI and Uniprot Product Information

• NCBI GI #: 6981652
• NCBI GeneID: 25085
• NCBI Accession #: NP_036872.1
• NCBI GenBank Nucleotide #: NM_012740.3
• UniProt Accession #: P04177
• Molecular Weight: 60
• NCBI Official Full Name: tyrosine 3-monooxygenase
• NCBI Official Synonym Full Names: tyrosine hydroxylase
• NCBI Official Symbol: Th
• NCBI Official Synonym Symbols: The
• NCBI Protein Information: tyrosine 3-monooxygenase; tyrosine 3-hydroxylase
• UniProt Protein Name: Tyrosine 3-monooxygenase
• UniProt Gene Name: Th
• UniProt Synonym Gene Names: TH
• UniProt Entry Name: TY3H_RAT

NCBI Description

catalyzes the rate limiting step in catecholamine biosynthesis; may be involved in response to hypoxia [RGD, Feb 2006]

Uniprot Description

Function: Plays an important role in the physiology of adrenergic neurons.

Catalytic activity: L-tyrosine + tetrahydrobiopterin + O2 = L-dopa + 4a-hydroxytetrahydrobiopterin.

Cofactor: Fe2+ ion.

Enzyme regulation: Phosphorylation leads to an increase in the catalytic activity.

Pathway: Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.

Subunit structure: Homotetramer.

Post-translational modification: In vitro, phosphorylation of Ser-19 increases the rate of Ser-40 phosphorylation, which results in enzyme opening and activation.

Sequence similarities: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.