Rabbit anti-Rat, mouse Potassium Channel, Voltage-Gated, Kv3.1 Subunit (Ser503) Polyclonal Antibody | anti-KCNC1 antibody

Anti-Phospho-Ser503 Kv3.1, Voltage-Gated, Potassium Channel

Cat. Num. AAA502105

• Gene Names: Kcnc1; Kv4; Kv3.1; KShIIIB; NGK2-KV4
• Reactivity: Rat, mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Synonyms: Potassium Channel; Voltage-Gated; Kv3.1 Subunit (Ser503); Polyclonal Antibody; Anti-Phospho-Ser503 Kv3.1; anti-KCNC1 antibody

• Host: Rabbit
• Reactivity: Rat, mouse
• Clonality: Polyclonal
• Specificity: Specific for the ~100k Kv3.1 voltage-gated potassium channel protein phosphorylated at Ser503.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 585

• Applicable Applications for anti-KCNC1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000; IHC (frozen sections; unpublished observations): 1:1000
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser503 of the voltage-gated potassium channel Kv3.1, conjugated to keyhole limpet hemocyanin (KLH).
• Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser503 conjugated to KLH
• Immunogen Species: Rat
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat and mouse tissue.
• Biological Significance: Voltage-gated K+ channels are important determinants of neuronal membrane excitability. Moreover, differences in K+ channel expression patterns and densities contribute to the variations in action potential waveforms and repetitive firing patterns evident in different neuronal cell types (Maletic-Savatic et al., 1995; Pongs, 1999; Blaine and Ribera, 1998; Burger and Ribera, 1996). The Kv3.1 potassium channel is expressed at high levels in neurons that characteristically fire rapid trains of action potentials (Gan et al., 1999).Particularly high levels of this channel are found in neurons of the auditory brainstem. These neurons appear to participate in neural circuits that determine the intensity and timing of auditory stimuli and use this information to determine the location of sounds in space (von Hehn et al., 2004).
• Preparation and Storage: For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Testing Data

Related Product Information for anti-KCNC1 antibody

Affinity purified rabbit polyclonal antibody

References

• Blaine JT, Ribera AB (1998) Heteromultimeric potassium channels formed by members of the Kv2 subfamily. J Neurosci 18:9585-9593.
• Burger C, Ribera AB (1996) Xenopus spinal neurons express Kv2 potassium channel transcripts during embryonic development. J Neurosci 16:1412-1421. Gan L, Hahn SJ, Kaczmarek LK (1999) Cell type-specific expression of the Kv3.1 gene is mediated by a negative element in the 5' untranslated region of the Kv3.1 promoter. J Neurochem 73:1350-1362.
• Maletic-Savatic M, Lenn NJ, Trimmer JS (1995) Differential spatiotemporal expression of K+ channel polypeptides in rat hippocampal neurons developing in situ and in vitro. J Neurosci 15:3840-3851.
• Pongs O (1999) Voltage-gated potassium channels: from hyperexcitability to excitement. FEBS Lett 452:31-35.
• von Hehn CA, Bhattacharjee A, Kaczmarek LK (2004) Loss of Kv3.1 tonotopicity and alterations in cAMP response element-binding protein signaling in central auditory neurons of hearing impaired mice. J Neurosci 24:1936-1940.

NCBI and Uniprot Product Information

• NCBI GI #: 6981122
• NCBI GeneID: 25327
• NCBI Accession #: NP_036988.1
• NCBI GenBank Nucleotide #: NM_012856.1
• UniProt Accession #: P25122
• Molecular Weight: 100
• NCBI Official Full Name: potassium voltage-gated channel subfamily C member 1
• NCBI Official Synonym Full Names: potassium voltage gated channel, Shaw-related subfamily, member 1
• NCBI Official Symbol: Kcnc1
• NCBI Official Synonym Symbols: Kv4; Kv3.1; KShIIIB; NGK2-KV4
• NCBI Protein Information: potassium voltage-gated channel subfamily C member 1; NGK2; RAW2; potassium channel gene 1; voltage-gated potassium channel subunit Kv4; voltage-gated potassium channel subunit Kv3.1
• UniProt Protein Name: Potassium voltage-gated channel subfamily C member 1
• Protein Family: Potassium voltage-gated channel subfamily
• UniProt Gene Name: Kcnc1
• UniProt Entry Name: KCNC1_RAT

NCBI Description

voltage gated potassium channel; functions as a delayed rectifier K+ channel [RGD, Feb 2006]

Uniprot Description

Function: This protein mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

Subunit structure: Heteromultimer with KCNG3, KCNG4 and KCNV2

By similarity.

Subcellular location: Membrane; Multi-pass membrane protein.

Tissue specificity: Brain.

Domain: The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.The tail may be important in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.

Sequence similarities: Belongs to the potassium channel family. C (Shaw) (TC 1.A.1.2) subfamily. Kv3.1/KCNC1 sub-subfamily. [View classification]