Goat anti-Mouse CD289 Polyclonal Antibody | anti-CD289 antibody

GOAT ANTI HUMAN CD289 (N-TERMINAL)

Cat. Num. AAA223954

• Gene Names: TLR9; CD289
• Reactivity: Mouse
• Applications: Immunohistochemistry, Western Blot
• Synonyms: CD289; Polyclonal Antibody; GOAT ANTI HUMAN CD289 (N-TERMINAL); anti-CD289 antibody

• Host: Goat
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item specifically recognises an epitope within the N-terminal (NT) region of CD289, otherwise known as Toll-like receptor 9 (TLR9), a type I transmembrane glycoprotein and member of the toll-like receptor family, which triggers MyD88 and TRAF6, resulting in NF-kappaB activation, cytokine secretion and the inflammatory response. CD289 acts as a receptor for unmethylated cytidine-phosphate-guanosine dinucleotides in bacterial DNA (CpG-DNA), playing a primary role in pathogen recognition and activation of innate immunity. This item is reported as suitable for use in immunocytochemistry on acetone fixed cells.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 1032

• Applicable Applications for anti-CD289 antibody: Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Immunohistology - Paraffin: Minimum Dilution: 1:250; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; Western Blotting: Minimum Dilution: 1:1000
• Perservative Stabilisers: 0.1% Sodium Azide (NaN3)
• Immunogen: Synthetic peptide SLKYNNLTVVPRNLPSSLEYLLLSYNRIVKL corresponding to amino acids 205-235 within the N-terminal domain of human CD289.; Histology Positive Control Tissue
• Antiserum Preparation: Antiserum to human CD289 (NT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Staining of paraffin embedded human spleen with Goat anti Human CD289 (MBS223954))

Testing Data #2

(Staining of paraffin embedded human spleen with Goat anti Human CD289 (MBS223954))

Related Product Information for anti-CD289 antibody

MBS223954 specifically recognises an epitope within the N-terminal (NT) region of CD289, otherwise known as Toll-like receptor 9 (TLR9), a type I transmembrane glycoprotein and member of the toll-like receptor family, which triggers MyD88 and TRAF6, resulting in NF-kappaB activation, cytokine secretion and the inflammatory response. CD289 acts as a receptor for unmethylated cytidine-phosphate-guanosine dinucleotides in bacterial DNA (CpG-DNA), playing a primary role in pathogen recognition and activation of innate immunity. MBS223954 is reported as suitable for use in immunocytochemistry on acetone fixed cells.

NCBI and Uniprot Product Information

• NCBI GI #: 8394456
• NCBI GeneID: 54106
• NCBI Accession #: NP_059138.1
• NCBI GenBank Nucleotide #: NM_017442.3
• UniProt Accession #: Q9NR96; Q6UVZ2; Q9HD68; Q9HD69; Q9HD70; Q9NYC2; Q9NYC3; B3Y661; D1CS56
• Molecular Weight: 114,237 Da
• NCBI Official Full Name: toll-like receptor 9
• NCBI Official Synonym Full Names: toll-like receptor 9
• NCBI Official Symbol: TLR9
• NCBI Official Synonym Symbols: CD289
• NCBI Protein Information: toll-like receptor 9
• UniProt Protein Name: Toll-like receptor 9
• UniProt Gene Name: TLR9
• UniProt Entry Name: TLR9_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response. [provided by RefSeq, Jul 2008]

Uniprot Description

TLR9: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Controls lymphocyte response to Helicobacter infection. Interacts with MYD88 via their respective TIR domains. Interacts (via transmembrane domain) with UNC93B1. Interacts with CD300LH; the interaction may promote full activation of TLR9-triggered innate responses. Interacts with BTK. Interacts with CNPY3 and HSP90B1; this interaction is required for proper folding in the endoplasmic reticulum. Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre- dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver. Belongs to the Toll-like receptor family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; endoplasmic reticulum; basolateral plasma membrane; lysosome; apical plasma membrane; cytoplasm; plasma membrane; extracellular region; integral to membrane; endosome membrane; endosome

Molecular Function: interleukin-1 receptor binding; siRNA binding; transmembrane receptor activity

Biological Process: positive regulation of JNK activity; regulation of cytokine secretion; positive regulation of interleukin-12 production; maintenance of gastrointestinal epithelium; positive regulation of JNK cascade; positive regulation of NF-kappaB import into nucleus; positive regulation of interferon-alpha biosynthetic process; positive regulation of interleukin-18 production; defense response to Gram-negative bacterium; positive regulation of interleukin-10 production; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; response to molecule of bacterial origin; positive regulation of interferon-beta production; negative regulation of interleukin-8 production; negative regulation of interleukin-6 production; inflammatory response; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of toll-like receptor signaling pathway; positive regulation of chemokine production; MyD88-dependent toll-like receptor signaling pathway; inhibition of NF-kappaB transcription factor; defense response to bacterium; positive regulation of interferon-beta biosynthetic process; insulin receptor signaling pathway; toll-like receptor signaling pathway; negative regulation of toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; I-kappaB phosphorylation; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of inflammatory response; positive regulation of interferon-gamma biosynthetic process