Rabbit anti-Human, Rat TIRAP Polyclonal Antibody | anti-TIRAP antibody

RABBIT ANTI TIRAP

Cat. Num. AAA221757

• Gene Names: Tirap; Mal; Wyatt; Tlr4ap; AA407980; C130027E04Rik
• Reactivity: Human, Rat
• Applications: Immunohistochemistry, Western Blot
• Synonyms: TIRAP; Polyclonal Antibody; RABBIT ANTI TIRAP; anti-TIRAP antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item is specific for mouse TIRAP (toll-interleukin 1 receptor domain containing adaptor protein), also known as Mal. ; ; TIRAP is one of a family of TIR domain-containing proteins which serve as adaptor proteins for the Toll-like receptors during the innate immune response. TIRAP is reported to participate in the MyD88-dependent signaling pathways shared by TLR2 and TLR4.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 249

• Applicable Applications for anti-TIRAP antibody: Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.; Western Blotting: Minimum Dilution: 0.5ug/ml; Maximum Dilution: 2.0ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide
• Immunogen: Synthetic peptide corresponding to a region within the C-Terminus of mouse TIRAP.; Buffer Solution
• Antiserum Preparation: Antisera to mouse TIRAP were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of human heart lysate probed with Rabbit anti Mouse TIRAP (MBS221757) at 0.5(A), 1(B) and 2(C) ug/ml)

Testing Data #2

(Immunohistochemical staining of human heart with Rabbit anti Mouse TIRAP (MBS221757))

Related Product Information for anti-TIRAP antibody

MBS221757 is specific for mouse TIRAP (toll-interleukin 1 receptor domain containing adaptor protein), also known as Mal. TIRAP is one of a family of TIR domain-containing proteins which serve as adaptor proteins for the Toll-like receptors during the innate immune response. TIRAP is reported to participate in the MyD88-dependent signaling pathways shared by TLR2 and TLR4.

NCBI and Uniprot Product Information

• NCBI GI #: 295390024
• NCBI GeneID: 117149
• NCBI Accession #: NP_473437.2
• NCBI GenBank Nucleotide #: NM_054096.2
• UniProt Accession #: Q99JY1; Q91ZW0
• Molecular Weight: 26,035 Da
• NCBI Official Full Name: toll/interleukin-1 receptor domain-containing adapter protein
• NCBI Official Synonym Full Names: toll-interleukin 1 receptor (TIR) domain-containing adaptor protein
• NCBI Official Symbol: Tirap
• NCBI Official Synonym Symbols: Mal; Wyatt; Tlr4ap; AA407980; C130027E04Rik
• NCBI Protein Information: toll/interleukin-1 receptor domain-containing adapter protein; TIR domain-containing adapter protein; Toll-like receptor 4 adaptor protein; adaptor protein Wyatt; myD88 adapter-like protein
• UniProt Protein Name: Toll/interleukin-1 receptor domain-containing adapter protein
• Protein Family: Toll/interleukin-1 receptor domain-containing adapter protein
• UniProt Gene Name: Tirap
• UniProt Synonym Gene Names: Mal; TIR domain-containing adapter protein
• UniProt Entry Name: TIRAP_MOUSE

Uniprot Description

TIRAP: an adapter protein involved in the TLR4 signaling pathway in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, ERK1, ERK2 and JNK, resulting in cytokine secretion and the inflammatory response. Homodimerizes. Also forms heterodimers with MyD88. Binds to TLR4 and IRAK2 via their respective TIR domains. Binds to PKR and TBK1. Does not interact with IRAK1, nor TLR9. Genetic variations in TIRAP can influence susceptibility or resistance to infectious diseases, including invasive pneumococcal disease, malaria and tuberculosis. Three isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Cellular Component: nucleoplasm; intercellular bridge; membrane; endocytic vesicle; cytoplasm; plasma membrane; peroxisome; intracellular

Molecular Function: protein binding; phosphatidylinositol-4,5-bisphosphate binding; protein homodimerization activity; protein kinase C binding; protein heterodimerization activity

Biological Process: I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-12 production; positive regulation of interleukin-6 biosynthetic process; positive regulation of JNK cascade; response to lipopolysaccharide; signal transduction; activation of JNK activity; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; toll-like receptor 5 signaling pathway; cell surface receptor linked signal transduction; positive regulation of interleukin-15 production; positive regulation of innate immune response; positive regulation of B cell proliferation; inflammatory response; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of toll-like receptor 3 signaling pathway; immune system process; positive regulation of toll-like receptor 4 signaling pathway; positive regulation of tumor necrosis factor production; regulation of interleukin-15 production; positive regulation of toll-like receptor 2 signaling pathway; regulation of innate immune response; defense response to Gram-positive bacterium; response to bacterium; innate immune response; myeloid cell differentiation; regulation of interferon-beta production