Rabbit ST2 Polyclonal Antibody | anti-ST2 antibody

RABBIT ANTI MOUSE ST2 (N-TERMINAL)

Cat. Num. AAA221876

• Gene Names: Il1rl1; T1; St2; DER4; Ly84; ST2L; Fit-1; T1/ST2; St2-rs1
• Applications: Western Blot
• Synonyms: ST2; Polyclonal Antibody; RABBIT ANTI MOUSE ST2 (N-TERMINAL); anti-ST2 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 337

• Applicable Applications for anti-ST2 antibody: Western Blot (WB)
• Application Notes: Western Blot: This item detecs a band of approximately 45kDa in mouse kidney cell lysates. (Predicted Mwt. 62 kDa).; Western Blotting: Minimum Dilution: 1; Maximum Dilution: 2ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide
• Immunogen: A 16 amino acid synthetic peptide located near mouse ST2 amino-terminus. This peptide is common to the three known isoforms of ST2.
• Buffer Solution: Phosphate buffered saline
• Antiserum Preparation: Antisera to mouse ST2 (NT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of mouse kidney lysate probed with Rabbit anti Mouse ST2 (MBS221876) in the absence (A) or presence (B) of blocking peptide)

Testing Data #2

(Immunocytochemical staining of mouse kidney with Rabbit anti Mouse ST2 (MBS221876))

Related Product Information for anti-ST2 antibody

Rabbit anti Mouse ST2 (N-Terminal) antibody recognizes an epitope within the N-Terminal region (NT) of mouse ST2, a member of the Toll-interleukin 1 receptor (TIR) superfamily, expressed by mast cells and TH2 helper cells, but not by TH1 effector cells. The majority of TIR superfamily members, which includes the Interleukin-1 receptor (IL-1R1), Toll-like receptors (TLRs) and the cytosolic adaptor proteins MyD88, TIRAP, Trif and TRAM, initiate innate immunity through the ultimate activation of the transcription factor NF-kappaB and are inducers of the inflammatory response. In contrast ST2, which itself is unable to activate NF-kappaB, has been identified as a negative regulator of IL-1R1 and TLR4 signaling by sequestering MyD88 and TIRAP in line with its role as a crucial receptor in TH2-cell mediated responses (Brint et al. 2004 and Coyle et al. 1999).

NCBI and Uniprot Product Information

• NCBI GI #: 71037395
• NCBI GeneID: 17082
• NCBI Accession #: NP_001020773.1
• NCBI GenBank Nucleotide #: NM_001025602.3
• UniProt Accession #: P14719; Q05208
• NCBI Official Full Name: interleukin-1 receptor-like 1 isoform a
• NCBI Official Synonym Full Names: interleukin 1 receptor-like 1
• NCBI Official Symbol: Il1rl1
• NCBI Official Synonym Symbols: T1; St2; DER4; Ly84; ST2L; Fit-1; T1/ST2; St2-rs1
• NCBI Protein Information: interleukin-1 receptor-like 1; lymphocyte antigen 84
• UniProt Protein Name: Interleukin-1 receptor-like 1
• Protein Family: High affinity sulfate transporter
• UniProt Gene Name: Il1rl1
• UniProt Synonym Gene Names: Ly84; St2; Ste2
• UniProt Entry Name: ILRL1_MOUSE

Uniprot Description

IL1RL1: receptor for IL33. Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of ERK1 and/or ERK2, p38-alpha, and JNK1. Apparently promotes the activation and differentiation of T-helper 2 cells. Belongs to the interleukin-1 receptor family. Highly expressed in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine. Three isoforms of the human protein are produced by alternative splicing. Isoform A is prevalently expressed in the lung, testis, placenta, stomach and colon. Isoform B is more abundant in the brain, kidney and the liver. Isoform C is not detected in brain, heart, liver, kidney and skeletal muscle.

Protein type: Membrane protein, integral; Receptor, cytokine

Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; membrane; plasma membrane; extracellular region; integral to membrane; external side of plasma membrane

Molecular Function: interleukin-1 receptor activity

Biological Process: negative regulation of cell proliferation; negative regulation of T-helper 1 type immune response; negative regulation of interferon-gamma production; negative regulation of I-kappaB kinase/NF-kappaB cascade; signal transduction; positive regulation of interleukin-5 production; positive regulation of inflammatory response; positive regulation of macrophage activation