Rabbit anti-Human Treponema pallidum Polyclonal Antibody

Treponema pallidum

Cat. Num. AAA373080

• Reactivity: Human
• Applications: Immunohistochemistry
• Synonyms: Treponema pallidum; Polyclonal Antibody; anti-Treponema pallidum antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Treponema pallidumid a purified immunoglobulin fraction of rabbit antiserum that is filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

• Applicable Applications for anti-Treponema pallidum antibody: Immunohistochemistry (IHC) Formalin/Paraffin
• Application Notes: Due to inherent variability present in immunohistochemical procedures (including fixation time of tissues, dilution factor of antibody, retrieval method utilized and incubation time), optimal performance should be established through the use of positive and negative controls. Results should be interpreted by a qualified medical professional.
• Immunogen: Purified treponema pallidum
• Localization: Cell Wall
• Control: Infected tissue
• Reactivity Note: Paraffin, Frozen
• Precautions: 1. For professional users only. Results should be interpreted by a qualified medical professional.; 2. This product contains <0.1% sodium azide (NaN₃) as a preservative. Ensure proper handling procedures are used with this reagent.; 3. Always wear personal protective equipment such as laboratory coat, goggles and gloves when handling reagents.; 4. Dispose of unused solution with copious amount of water.; 5. Do not ingest reagent. If reagent is ingested, seek medical advice immediately.; 6. Avoid contact with eyes. If contact occurs, flush with large quantities of water.; 7. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar).; 8. For additional safety information refer to Safety Data Sheet for this product.; 9. For complete recommendations for handling biological specimens, please refer to the CDC document, “Guidelines for Safe Work Practices in Human and AMDLLaboratories”.
• Specimen Preparation: Paraffin sections: The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation for best results. Pre-treatment of tissues with heat-induced epitode retrieval (HIER) is recommended using ImmunoDNA retriever with citrate, ImmunoDNA retriever with EDTA, or ImmunoDNA Digestor. See reverse side for complete protocol. Tissue should remain hydrated via use of ImmunoDNA washer solutions (Contact Tech Support).
• Frozen Sections and Cell Preparation: The antibody can be used for labeling acetone-fixed frozen sections and acetone-fixed cell preparations.
• Mounting Protocols: For detail instructions using biodegradable permanent mounting media such as XyGreen PermaMounter or organic solvent based resin such as Permamounter contact Technical Support.
• Preparation and Storage: Store at 2-8 °C.

Dilution Information

Immunohistochemistry (IHC)

(IHC of Treponema pallidum on an FFPE Infected Skin Tissue)

Quality Control

(This antibody has been quality control tested by Immunohistochemistry as follows)

Related Product Information for anti-Treponema pallidum antibody

Treponema pallidum is a spirochaete bacterium. The treponemes have a cytoplasmic and an outer membrane. The shape of T. pallidum is flat and wavy, unlike the other spirochetes, which are helical. Using light microscopy, treponemes are only visible using dark field illumination. They are Gram negative, but some regard them too thin to be Gram stained. T. pallidum is a motile spirochaete that is generally acquired by close sexual contact, entering the host via breaches in squamous or columnar epithelium. The organism can also be transmitted to a fetus by transplacental passage during the later stages of pregnancy, giving rise to congenital syphilis. The helical structure of T. pallidum allows it to move in a corkscrew motion through a viscous medium such as mucus. It gains access to the host's blood and lymph systems through tissue and mucous membranes.

Detection of Treponema pallidum can be difficult, and the correct identification of secondary syphilis is critical. identification of syphilis is usually based on clinical presentation, dark-field microscope analysis, and serological tests. T. pallidum can be also evidenced by Immunohistochemistry in up to 90% of the samples with the bacteria located in the epidermis and the upper dermis of formalin-fixed paraffin-embedded tissues.