Testing Data (TD)
SV-40 Slide
SV-40 Cell Line Array
Synonyms
SV-40; SV-40 Cell Line Array; SV-40 slide
Form/Format
Five Infectious Disease CLMA’s with 2 or 9 – 2mm cores each, mounted on Hydrophilic Plus Slides are provided in a plastic mailer.
Precautions
1. For professional users only. Results should be interpreted by a qualified medical professional.
2. Ensure proper handling procedures are used with reagent.
3. Always wear personal protective equipment such as laboratory coat, goggles, and gloves when handling reagents.
4. Dispose of unused material according to local and federal regulations.
5. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar).
6. For additional safety information refer to Safety Data Sheet for this product.
7. For complete recommendations for handling biological specimens please refer to the CDC document, “Guidelines for Safe Work Practices in Human and AMDL” (see References in this document)
Staining Procedure
1. Deparaffinize, dehydrate and rehydrate tissues.
2. Subject tissues to heat induced epitope retrieval (HIER) using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate (please inquire), or ImmunoDNA Retriever EDTA (please inquire).
3. Any of three heating methods may be used:
a. TintoRetriever Pressure Cooker or Equivalent Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place on trivet in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature.
b. TintoRetriever PT Module or Water Bath Method Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes.
c. Conventional Steamer Method Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a steamer, cover and steam for 30-60 minutes.
4. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.
5. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions.
6. Wash slides with ImmunoDNA washer or DI water.
7. Continue IHC staining protocol. Wash slides between each step with ImmunoDNA washer solution.
Preparation and Storage
Store at 2C to 8°C.
Testing Data (TD)
Testing Data (TD)
Testing Data (TD)
Testing Data (TD)
Related Product Information for SV-40 slide
The Infectious Disease Cell Line Microarray consists of either 2 or 9- 2mm cores of formalin-fixed paraffin-embedded infected cell lines, which were assembled in array fashion to allow multiplex molecular pathology analysis and validation of infectious disease reagents, or to be used as controls for Immunohistochemistry and/or in situ hybridization (CISH and FISH) applications.The 9-core Infectious Disease Cell Line Microarray consists of all positive (+) cell lines listed below, as well as one negative (-) core. The 2-core Infectious Disease Cell Line Microarrays include one positive (+) and one negative (-) core.The following are the Infectious Disease Cell Lines offered: Adenovirus (ADV), Cytomegalovirus (CMV), Herpes Simplex Virus (HSV), Varicella Zoster Virus (VZV), Epstein Barr Virus (EBV), Hepatitis B Virus (HBV), HHV-8, SV-40, and Helicobacter pylori (HPy).
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Product Notes
The SV-40 (Catalog #AAA373156) is a Slide and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SV-40, Slide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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