Mouse CD20 Monoclonal Antibody | anti-CD20 antibody

CD20

Cat. Num. AAA370021

• Gene Names: MS4A1; CD20
• Reactivity: Human, Canine, Feline, Mouse
• Synonyms: CD20; Monoclonal Antibody; anti-CD20 antibody

• Host: Mouse
• Reactivity: Human, Canine, Feline, Mouse
• Clonality: Monoclonal
• Isotype: IgG2a/K
• Clone Number: L26
• Form/Format: CD20 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

• Application Notes: Intended Use: This antibody is intended for use in Immunohistochemical applications on formalin fixed paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations. Interpretations of results should be performed by a qualified medical professional.
• Immunogen: Human Tonsil B cells
• Control Slides: MBS370021; 5 slides
• Localization: Membranous
• Control: Tonsil, Lymph Node
• Reactivity Note: Paraffin, Frozen
• Precautions: 1. For professional users only. Results should be interpreted by a qualified medical professional.; 2. This product contains <0.1% sodium azide (NaN₃) as a preservative. Ensure proper handling procedures are used with this reagent.; 3. Always wear personal protective equipment such as laboratory coat, goggles and gloves when handling reagents.; 4. Dispose of unused solution with copious amount of water.; 5. Do not ingest reagent. If reagent is ingested, seek medical advice immediately.; 6. Avoid contact with eyes. If contact occurs, flush with large quantities of water.; 7. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar).; 8. For additional safety information refer to Safety Data Sheet for this product.; 9. For complete recommendations for handling biological specimens, please refer to the CDC document, “Guidelines for Safe Work Practices in Human and AMDL”.
• Specimen Preparation: Paraffin sections: The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation to ensure best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using ImmunoDNA Retriever with Citrate, ImmunoDNA Retriever with EDTA or ImmunoDNA Digestor. Tissue should remain hydrated via use of Immuno/DNA Washer solutions. ; Frozen sections and cell preparations: The antibody can be used for labeling acetone-fixed frozen sections and acetone-fixed cell preparations.
• Staining Procedure: 1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positive charged slides such as Hydrophilic Plus Slides. ; 2. Air dry for 2 hours at 58° C. ; 3. Deparaffinize, dehydrate and rehydrate tissues. ; 4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA ; 5. Any of three heating methods may be used:; ; a. TintoRetriever Pressure Cooker or Equivalent; Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature.; b. TintoRetriever PT Module or Water Bath Method; Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes. ; c. Conventional Steamer Method; Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes.; ; 6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.; 7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions. ; 8. Wash slides with IHC wash buffer or DI water. ; 9. Continue IHC staining protocol.
• Product Limitations: Due to inherent variability present in immunohistochemical procedures (including fixation time of tissues, dilution factor of antibody, retrieval method utilized and incubation time), optimal performance should be established through the use of positive and negative controls. Results should be interpreted by a medical professional.
• Mounting Protocols: For detailed instructions using biodegradable permanent mounting media such as XyGreen PermaMounter or organic solvent based resin such as PermaMounter please contact Tech Support.
• Preparation and Storage: Storage: Store at 2-8°C (Control Slides: Store at 20-25°C); ; Stability: This product is stable up to the expiration date on the product label. Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropriately when not in use, and avoid prolonged exposure to room temperature conditions.

Immunohistochemistry (IHC)

(Inset: IHC of CD20 on a FFPE Colon Tissue)

Testing Data

Abbreviated Immunohistochemical Protocol

Testing Data

Related Product Information for anti-CD20 antibody

CD20 is a transmembrane, non-glycosylated protein expressed on B-cell precursors and mature B-cells, but lost following differentiation into plasma cells. This antibody does not cross-react with non-hematopoietic neoplasms. CD20 (B-cell Pan) reacts with a membrane antigen present in B-cells. This antibody strongly recognizes Reed-Sternberg cells predominant in Hodgkin's disease. Since no staining of histiocytes or plasma cells has been observed and CD20 has not been detected in T-cell malignancies, it is a very strong marker of B-cell Lymphomas. B-cell Panmarker recognizes a formalin-resistant intracytoplasmic antigen.

NCBI and Uniprot Product Information

• NCBI GI #: 78183404
• NCBI GeneID: 485430
• UniProt Accession #: Q3C2E2
• NCBI Official Full Name: CD20
• NCBI Official Symbol: MS4A1
• NCBI Official Synonym Symbols: CD20
• NCBI Protein Information: B-lymphocyte antigen CD20; membrane-spanning 4-domains subfamily A member 1
• UniProt Protein Name: B-lymphocyte antigen CD20
• Protein Family: CD209 antigen
• UniProt Gene Name: MS4A1
• UniProt Synonym Gene Names: CD20
• UniProt Entry Name: CD20_CANFA

Uniprot Description

Function: This protein may be involved in the regulation of B-cell activation and proliferation

Subcellular location: Membrane; Multi-pass membrane protein

Tissue specificity: Expressed in PBMCs and lymph node from healthy dogs, in B-cells of canine lymphoma, but not in T-cell lymphoma cells and non-T and non-B-cell lymphoma cells. Ref.1

Post-translational modification: Phosphorylated. Might be functionally regulated by protein kinase(s)

Sequence similarities: Belongs to the MS4A family.