Mouse anti-Human Epstein Barr Virus, LMP-1 Monoclonal Antibody

Epstein Barr Virus, LMP-1

Cat. Num. AAA370066

• Reactivity: Human
• Synonyms: Epstein Barr Virus; LMP-1; Monoclonal Antibody; anti-Epstein Barr Virus; LMP-1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: CS1-4
• Form/Format: EBV antibody is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

• Application Notes: Product Limitations: Due to inherent variability present in immunohistochemical procedures (including fixation time of tissues, dilution factor of antibody, retrieval method utilized and incubation time), optimal performance should be established through the use of positive and negative controls. Results should be interpreted by a qualified medical professional.
• Immunogen: Recombinant fusion protein of bacterial beta-galactosidase and EBV LMP-1.
• Reactivity Note: Paraffin, Frozen
• Localization: Cytoplasmic
• Control: EBV Infected Tissue; Hodgkins's Lymphoma
• Precautions: 1. For professional users only. Results should be interpreted by a qualified medical; professional. 2. This product contains <0.1% sodium azide (NaN?) as a preservative. Ensure proper handling procedures are used with this reagent.; 3. Always wear personal protective equipment such as laboratory coat, goggles and gloves when handling reagents.; 4. Dispose of unused solution with copious amount of water.; 5. Do not ingest reagent. If reagent is ingested, seek medical advice immediately.; 6. Avoid contact with eyes. If contact occurs, flush with large quantities of water.; 7. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar).; 8. For additional safety information refer to Safety Data Sheet for this product.; 9. For complete recommendations for handling biological specimens, please refer to the CDC document, “Guidelines for Safe Work Practices in Human and AMDL” (see References in this document).
• Specimen Preparation: Paraffin sections: The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation for best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using ImmunoDNA Retriever with Citrate, ImmunoDNA Retriever with EDTA or ImmunoDNA Digestor. Tissue should remain hydrated via use of Immuno/DNA Washer solutions.
• Frozen sections and cell preparations: The antibody can be used for labeling acetone-fixed frozen sections and acetone-fixed cell preparations.
• Mounting Protocols: For detailed instructions using biodegradable permanent mounting media such as XyGreen PermaMounter or organic solvent based resin such as PermaMounter.
• Preparation and Storage: Store at 2°C to 8°C.; Stability : This product is stable up to the expiration date on the product label. Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropriately when not in use, and avoid prolonged exposure to room temperature conditions.

Dilution Information

Immunohistochemistry (IHC)

Quality Control Procedure

Related Product Information for anti-Epstein Barr Virus, LMP-1 antibody

The Epstein-Barr virus (EBV), also called Human Herpesvirus 4 (HHV-4), is a virus of the Herpes family, and is one of the most common viruses in humans. The virus can execute many distinct programs of gene expression, which can be broadly categorized as being lytic cycle or latent cycle. The lytic cycle, or productive infection, results in staged expression of several viral proteins with the ultimate objective of producing infectious virions. The latent cycle (lysogenic) programs are those that do not result in production of virions. A very limited, distinct set of viral proteins are produced during latent cycle infection. These include Epstein-Barr nuclear antigens EBNA-1, EBNA-2, EBNA-3A, EBNA-3B, EBNA-3C, EBNA-leader protein (EBNA-LP), latent membrane proteins LMP-1, LMP-2A and LMP-2B and the Epstein-Barr encoded RNAs (EBERs). In addition, EBV codes for at least twenty microRNAs which are expressed in latently infected cells.