Tas I Restriction Enzyme | tas restriction enzyme

Tas I

Cat. Num. AAA638449

• Purity: Molecular Biology Grade
• Synonyms: Tas I; tas restriction enzyme

• Purity/Purification: Molecular Biology Grade

• Compatible Ends: EcoRI, MunI, XapI
• pACYC177: 14
• pACYC184: 13
• Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.
• Lambda: 189
• Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with TasI, more than 90% of the DNA fragments can be ligated at a 5'-termini concentration of 3.4uM. More than 90% of these can be recut.
• M13mp18/19: 64
• pBR322: 8
• PhiX174: 25
• pTZ19R/U: 10
• pUC18/19: 7
• pUC57: 7
• Stability During Prolonged Incubation: A minimum of 0.3 units of enzyme are required for complete digestion of 1ug of lambda DNA in 16 hours at 65 degree C.
• Storage Buffer: 10mM Tris-HCl (pH 7.5 at 25 degree C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
• Thermal Inactivation: Enzyme is not inactivated by incubation at 80 degree C for 20min.
• Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 65 degree C in 50ul of assay buffer.
• Preparation and Storage: -20 degree C

Testing Data

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Related Product Information for tas restriction enzyme

5'-^A A T T-3'

Product Categories/Family for tas restriction enzyme

Molecular Biology; MB-Enzymes; Restriction

NCBI and Uniprot Product Information

• NCBI GI #: 9629928
• NCBI GeneID: 2717065
• NCBI Accession #: NP_056917.1
• NCBI GenBank Nucleotide #: NC_001871.1
• UniProt Accession #: O56862
• Molecular Weight: 23,124 Da
• NCBI Official Full Name: Tas
• NCBI Official Symbol: tas
• NCBI Protein Information: Bel 1; Tas
• UniProt Protein Name: Protein Bel-1
• Protein Family: Protein
• UniProt Gene Name: bel1
• UniProt Synonym Gene Names: taf; tas; Tas
• UniProt Entry Name: BEL1_FFV

Uniprot Description

Function: Transcriptional transactivator that activates the viral internal promoter (IP), thereby enhancing its own expression. This transactivation is repressed by nuclear factor I. Also transactivates the long terminal repeat (LTR) promoter, thereby inducing structural gene expression, initiating the late phase of infection. It is therefore a key regulator of viral gene expression. It directly binds to and activates DNA target sites of viral promoters and those of distinct cellular genes. Required for viral replication

By similarity.