Typical Testing Data/Standard Curve (for reference only)
Rat Dopamine Receptor D2 ELISA Kit | DRD2 elisa kit
Rat Dopamine Receptor D2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: DRD2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-DRD2 antibody and an DRD2-HRP conjugate. The assay sample and buffer are incubated together with DRD2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DRD2 concentration since DRD2 from samples and DRD2-HRP conjugate compete for the anti-DRD2 antibody binding site. Since the number of sites is limited, as more sites are occupied by DRD2 from the sample, fewer sites are left to bind DRD2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DRD2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the D2 subtype of the dopamine receptor. This G-protein coupled receptor inhibits adenylyl cyclase activity. A missense mutation in this gene causes myoclonus dystonia; other mutations have been associated with schizophrenia. Alternative splicing of this gene results in two transcript variants encoding different isoforms. A third variant has been described, but it has not been determined whether this form is normal or due to aberrant splicing. [provided by RefSeq, Jul 2008]
Uniprot Description
DRD2: Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Defects in DRD2 are associated with dystonia type 11 (DYT11); also known as alcohol-responsive dystonia. DYT11 is a myoclonic dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT11 is characterized by involuntary lightning jerks and dystonic movements and postures alleviated by alcohol. Inheritance is autosomal dominant. The age of onset, pattern of body involvement, presence of myoclonus and response to alcohol are all variable. Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR
Chromosomal Location of Human Ortholog: 11q23
Cellular Component: synaptic vesicle membrane; endocytic vesicle; integral to plasma membrane; axon; dendrite; postsynaptic density; plasma membrane; acrosome; dendritic spine; perikaryon; nerve terminal; lateral plasma membrane
Molecular Function: dopamine D2 receptor-like receptor activity; identical protein binding; ionotropic glutamate receptor binding; protein binding; protein homodimerization activity; potassium channel regulator activity; protein heterodimerization activity; dopamine binding; drug binding
Biological Process: response to nicotine; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of dopamine uptake; prepulse inhibition; response to toxin; positive regulation of receptor internalization; positive regulation of multicellular organism growth; thermoregulation; regulation of phosphoprotein phosphatase activity; adult walking behavior; dopamine metabolic process; dopamine receptor, adenylate cyclase inhibiting pathway; negative regulation of insulin secretion; protein localization; phosphatidylinositol metabolic process; negative regulation of blood pressure; response to drug; response to light stimulus; response to inactivity; positive regulation of neuroblast proliferation; cerebral cortex GABAergic interneuron migration; negative regulation of synaptic transmission, glutamatergic; regulation of sodium ion transport; arachidonic acid secretion; regulation of synaptic transmission, GABAergic; positive regulation of growth hormone secretion; G-protein coupled receptor internalization; reduction of cytosolic calcium ion concentration; activation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; regulation of long-term neuronal synaptic plasticity; synaptic transmission, dopaminergic; peristalsis; branching morphogenesis of a nerve; negative regulation of circadian sleep/wake cycle, sleep; response to morphine; locomotory behavior; behavioral response to ethanol; orbitofrontal cortex development; negative regulation of cell proliferation; synaptogenesis; behavioral response to cocaine; visual learning; adenohypophysis development; feeding behavior; nerve-nerve synaptic transmission; response to axon injury; circadian regulation of gene expression; negative regulation of dopamine secretion; response to iron ion; negative regulation of cell migration; associative learning; grooming behavior; positive regulation of cytokinesis; negative regulation of protein secretion; Wnt receptor signaling pathway; negative regulation of protein kinase B signaling cascade; striatum development; regulation of heart rate; regulation of potassium ion transport; regulation of cAMP metabolic process; negative regulation of adenylate cyclase activity; sensory perception of smell; response to amphetamine; regulation of dopamine uptake; auditory behavior; response to cocaine; cellular calcium ion homeostasis; regulation of systemic arterial blood pressure by neurological process; negative regulation of dopamine receptor signaling pathway; axonogenesis; pigmentation; long-term memory; release of sequestered calcium ion into cytosol; positive regulation of G-protein coupled receptor protein signaling pathway; response to hypoxia; regulation of synapse structural plasticity; dopamine receptor, phospholipase C activating pathway
Disease: Myoclonic Dystonia
Research Articles on DRD2
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Product Notes
The Rat DRD2 drd2 (Catalog #AAA724839) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA724839 ELISA Kit recognizes Rat DRD2. It is sometimes possible for the material contained within the vial of "Dopamine Receptor D2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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