Immunofluorescence (IF)
(Immunofluorescence staining of TPK1 in PC3 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human TPK1 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
Rabbit anti-Human TPK1 Polyclonal Antibody | anti-TPK1 antibody
Anti-TPK1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
TPK1; Polyclonal Antibody; Anti-TPK1 Antibody; Rabbit Polyclonal; TPK1 Antibody; Rabbit PAb; Antigen Affinity Purified; thiamin pyrophosphokinase 1; Anti-HTPK1 Antibody; Anti-PP20 Antibody; Anti-THMD5 Antibody; anti-TPK1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human TPK1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-TPK1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
ICC/IF: 1:500-1:2500
IP: 0.2-1uL/mg of lysate
ICC/IF: 1:500-1:2500
IP: 0.2-1uL/mg of lysate
Immunogen
E Coli-derived Human TPK1 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human TPK1 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of TPK1 in PC3 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human TPK1 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
Immunofluorescence (IF)
(Immunofluorescence staining of TPK1 in PC3 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human TPK1 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
Immunoprecipitation (IP)
(TPK1 was immunoprecipitated using: Lane A:0.5 mg H1299 Whole Cell Lysate 1 uL anti-TPK1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-TPK1 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa Observed band size :30 kDa)
Immunoprecipitation (IP)
(TPK1 was immunoprecipitated using: Lane A:0.5 mg H1299 Whole Cell Lysate 1 uL anti-TPK1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-TPK1 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa Observed band size :30 kDa)
Related Product Information for anti-TPK1 antibody
Thiamine pyrophosphokinase (TPK) produces thiamine pyrophosphate, a cofactor for a number of enzymes, including pyruvate dehydrogenase and 2-ketoglutarate dehydrogenase. Episodic encephalopathy type thiamine metabolism dysfunction (OMIM 614458) due to TPK1 mutations is a recently described rare disorder. The genomic variations in the fetal and maternal TPK1 gene could contribute to the variability of birth weight in normal humans.
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Product Notes
The TPK1 (Catalog #AAA8109512) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TPK1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TPK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:1000 ICC/IF: 1:500-1:2500 IP: 0.2-1uL/mg of lysate. Researchers should empirically determine the suitability of the TPK1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TPK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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