Rabbit anti-Human SSBP1 Polyclonal Antibody | anti-SSBP1 antibody

Anti-SSBP1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8109472

• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: SSBP1; Polyclonal Antibody; Anti-SSBP1 Antibody; Rabbit Polyclonal; SSBP1 Antibody; Rabbit PAb; Antigen Affinity Purified; single-stranded DNA binding protein 1; mitochondrial; Anti-Mt-SSB Antibody; Anti-mtSSB Antibody; Anti-SOSS-B1 Antibody; Anti-SSBP Antibody; anti-SSBP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human SSBP1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-SSBP1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; ICC/IF: 1:500-1:2500; IP: 1-2uL/mg of lysate
• Immunogen: E Coli-derived Human SSBP1 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human SSBP1 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-SSBP1 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:17 kDa Observed band size:15 kDa)

Immunofluorescence (IF)

(Immunofluorescence staining of SSBP1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human SSBP1 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

Immunoprecipitation (IP)

(SSBP1 was immunoprecipitated using: Lane A:0.5 mg U-251 MG Whole Cell Lysate 1 uL anti-SSBP1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-SSBP1 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 17 kDa Observed band size :15 kDa)

Related Product Information for anti-SSBP1 antibody

SSBP1 (Single-Stranded DNA Binding Protein 1) is a Protein Coding gene. The SSBP1 gene, located on 7q34, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, and frog. SSBP1 is a housekeeping gene involved in mitochondrial biogenesis. It is also a subunit of a single-stranded DNA (ssDNA)-binding complex involved in the maintenance of genome stability. Nuclear genes encode several proteins that function at the mtDNA replication fork, including mitochondrial single-stranded DNA-binding protein (SSBP1), which is a tetrameric protein that binds and protects single-stranded mtDNA (ssDNA). SSBP1 is widely expressed in the appendix, adrenal, and other tissues. Diseases associated with SSBP1 include Mitochondrial Dna Depletion Syndrome 7 and Autosomal Dominant Non-Syndromic Sensorineural Deafness Type Dfna.

References

Tian HP, et al. (2018) Single-stranded DNA-binding protein 1 abrogates cardiac fibroblast proliferation and collagen expression induced by angiotensin ii. Int Heart J 59 (6): 1398-1408.Piro-Megy C, et al. (2020) Dominant mutations in mtdna maintenance gene ssbp1 cause optic atrophy and foveopathy. J Clin Invest 130 (1): 143-156.Gustafson MA, et al. (2019) Mitochondrial single-stranded DNA binding protein novel de novo ssbp1 mutation in a child with single large-scale mtdna deletion (slsmd) clinically manifesting as pearson, kearns-sayre, and leigh syndromes. PLoS One 14 (9): e0221829.