Western Blot (WB)
(Anti-VTA1 rabbit polyclonal antibody at 1:500 dilution Lane A: H1299 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: Molt4 Whole Cell Lysate Lane D: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:34 kDa Observed band size:38 kDa)
Rabbit VTA1 Polyclonal Antibody | anti-VTA1 antibody
Anti-VTA1 Antibody, Rabbit Polyclonal
Predicted to React with: Pongo abelii (Species predicted to react based on 100% sequence homology)
Predicted to React with: Pongo abelii (Species predicted to react based on 100% sequence homology)
ICC/IF: 1:100-1:500
IP: 5-10uL/mg of lysate
Western Blot (WB)
(Anti-VTA1 rabbit polyclonal antibody at 1:500 dilution Lane A: H1299 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: Molt4 Whole Cell Lysate Lane D: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:34 kDa Observed band size:38 kDa)
Western Blot (WB)
(Anti-VTA1 rabbit polyclonal antibody at 1:500 dilution Lane A: H1299 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: Molt4 Whole Cell Lysate Lane D: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:34 kDa Observed band size:38 kDa)
Immunofluorescence (IF)
(Immunofluorescence staining of VTA1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human VTA1 polyclonal antibody (dilution ratio 1:500) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.)
Immunofluorescence (IF)
(Immunofluorescence staining of VTA1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human VTA1 polyclonal antibody (dilution ratio 1:500) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.)
Immunoprecipitation (IP)
(VTA1 was immunoprecipitated using: Lane A:0.5 mg H1299 Whole Cell Lysate 4 uL anti-VTA1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-VTA1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa Observed band size :39 kDa)
Immunoprecipitation (IP)
(VTA1 was immunoprecipitated using: Lane A:0.5 mg H1299 Whole Cell Lysate 4 uL anti-VTA1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-VTA1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa Observed band size :39 kDa)
NCBI and Uniprot Product Information
NCBI Description
C6ORF55 encodes a protein involved in trafficking of the multivesicular body, an endosomal compartment involved in sorting membrane proteins for degradation in lysosomes (Ward et al., 2005 [PubMed 15644320]).[supplied by OMIM, Mar 2008]
Uniprot Description
VTA1: Involved in the endosomal multivesicular bodies (MVB) pathway. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. Thought to be a cofactor of VPS4A/B, which catalyzes disassembles membrane-associated ESCRT-III assemblies. Involved in the sorting and down-regulation of EGFR. Involved in HIV-1 budding. Belongs to the VTA1 family.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 6q24.1
Cellular Component: endosome membrane; cytosol
Molecular Function: protein binding
Biological Process: protein transport; viral reproduction; viral infectious cycle; endosome transport
Research Articles on VTA1
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Product Notes
The VTA1 vta1 (Catalog #AAA8103734) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-VTA1 Antibody, Rabbit Polyclonal reacts with Human Predicted to React with: Pongo abelii (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's VTA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:2000 ICC/IF: 1:100-1:500 IP: 5-10uL/mg of lysate. Researchers should empirically determine the suitability of the VTA1 vta1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VTA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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