Rabbit NFYA Polyclonal Antibody | anti-NFYA antibody

Anti-NFYA Antibody, Rabbit Polyclonal

Cat. Num. AAA8100502

• Gene Names: NFYA; HAP2; CBF-A; CBF-B; NF-YA
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: NFYA; Polyclonal Antibody; Anti-NFYA Antibody; Rabbit Polyclonal; NFYA Antibody; Rabbit PAb; Antigen Affinity Purified; nuclear transcription factor Y subunit alpha; Anti-CBF-A Antibody; Anti-CBF-B Antibody; Anti-HAP2 Antibody; Anti-NF-YA Antibody; anti-NFYA antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human NFYA
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-NFYA antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; ICC/IF: 1:1500-1:50,000; IP: 0.2-1uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human NFYA
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human NFYA, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence staining of NFYA in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human NFYA polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus.)

Immunoprecipitation (IP)

(NFYA was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate1 uL anti-NFYA rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-NFYA rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 38 kDaObserved band size: 38 kDa)

Western Blot (WB)

(Anti-NFYA rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: Hela Whole Cell LysateLane C: NIH3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:37 kDaObserved band size:40 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-NFYA antibody

The increased expression of the alpha subunit of nuclear transcription factor Y (NFYA) is associated with the malignant phenotype of various tumors. NFYA may promote a malignant phenotype in OS cells, in part, by activating the FASN signaling pathway, which may represent a promising target for the management of OS.

NCBI and Uniprot Product Information

• NCBI GI #: 4505389
• NCBI GeneID: 4800
• NCBI Accession #: NP_002496.1
• NCBI GenBank Nucleotide #: NM_002505.4
• UniProt Accession #: P23511; Q8IXU0
• Molecular Weight: 36,877 Da
• NCBI Official Full Name: nuclear transcription factor Y subunit alpha isoform 1
• NCBI Official Synonym Full Names: nuclear transcription factor Y, alpha
• NCBI Official Symbol: NFYA
• NCBI Official Synonym Symbols: HAP2; CBF-A; CBF-B; NF-YA
• NCBI Protein Information: nuclear transcription factor Y subunit alpha; HAP2 CCAAT-binding protein; Transcription factor NF-Y, A subunit; CAAT box DNA-binding protein subunit A; CAAT-box DNA binding protein subunit A; nuclear transcription factor Y subunit A; CCAAT-binding transcr
• UniProt Protein Name: Nuclear transcription factor Y subunit alpha
• Protein Family: Nuclear transcription factor
• UniProt Gene Name: NFYA
• UniProt Synonym Gene Names: NF-YA
• UniProt Entry Name: NFYA_HUMAN

NCBI Description

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

NFYA: stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. Involved in cisplatin resistance. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: nucleoplasm; CCAAT-binding factor complex; nucleus

Molecular Function: protein binding; DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; rhythmic process; positive regulation of transcription from RNA polymerase II promoter; cellular lipid metabolic process