Rabbit TMEM38A Polyclonal Antibody | anti-TMEM38A antibody

Anti-TMEM38A Antibody, Rabbit Polyclonal

Cat. Num. AAA8108756

• Reactivity: Human, Cynomolgus
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: TMEM38A; Polyclonal Antibody; Anti-TMEM38A Antibody; Rabbit Polyclonal; TMEM38A Antibody; Rabbit PAb; Antigen Affinity Purified; transmembrane protein 38A; Anti-TRIC-A Antibody; Anti-TRICA Antibody; anti-TMEM38A antibody

• Host: Rabbit
• Reactivity: Human, Cynomolgus
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human TMEM38A
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-TMEM38A antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:2000-1:10,000; IHC-P: 1:50-1:200; ICC/IF: 1:100-1:500
• Immunogen: E Coli-derived Human TMEM38A fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human TMEM38A fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-TMEM38A rabbit polyclonal antibody at 1:5000 dilution Lane A: Jurkat Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:33 kDa Observed band size:36 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human TMEM38A in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human TMEM38A in cynomolgus cerebellum with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of TMEM38A in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human TMEM38A polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)

Related Product Information for anti-TMEM38A antibody

TMEM38A (Transmembrane Protein 38A) is a Protein Coding gene. The TMEM38A gene, located on 19p13.11, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, and frog. An important paralog of this gene is TMEM38B. TMEM38A is a monovalent cation channel required for the maintenance of rapid intracellular calcium release and may act as a potassium counter-ion channel that functions in synchronization with calcium release from intracellular stores. TMEM38A is widely expressed in the brain, stomach, and other tissues. Diseases associated with TMEM38A include Hyperalphalipoproteinemia 1 and Myopathy, X-Linked, With Postural Muscle Atrophy. 236 organisms have orthologs with the human gene TMEM38A.

References

Yamazaki D, et al. (2009) New molecular components supporting ryanodine receptor-mediated ca(2+) release: Roles of junctophilin and tric channel in embryonic cardiomyocytes. Pharmacol Ther 121 (3): 265-272.Shrestha N, et al. (2020) Tric-a shapes oscillatory ca2+ signals by interaction with stim1/orai1 complexes. PLoS Biol 18 (4): e3000700.Bailey SD, et al. (2010) Variation at the nfatc2 locus increases the risk of thiazolidinedione-induced edema in the diabetes reduction assessment with ramipril and rosiglitazone medication (dream) study. Diabetes Care 33 (10): 2250-2253.