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Western Blot (WB)
(Anti-SSR3 rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: U87 MG Whole Cell Lysate Lane D: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:21 kDa Observed band size:20 kDa)
Rabbit anti-Human SSR3 Polyclonal Antibody | anti-SSR3 antibody
Anti-SSR3 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
SSR3; Polyclonal Antibody; Anti-SSR3 Antibody; Rabbit Polyclonal; SSR3 Antibody; Rabbit PAb; Antigen Affinity Purified; signal sequence receptor; gamma (translocon-associated protein gamma); Anti-TRAPG Antibody; anti-SSR3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human SSR3
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-SSR3 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:50-1:200
ICC/IF: 1:100-1:500
IP: 0.5-2uL/mg of lysate
IHC-P: 1:50-1:200
ICC/IF: 1:100-1:500
IP: 0.5-2uL/mg of lysate
Immunogen
E Coli-derived Human SSR3 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human SSR3 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-SSR3 rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: U87 MG Whole Cell Lysate Lane D: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:21 kDa Observed band size:20 kDa)
Western Blot (WB)
(Anti-SSR3 rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: U87 MG Whole Cell Lysate Lane D: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:21 kDa Observed band size:20 kDa)
Immunohistochemistry (IHC)
(Immunochemical staining of human SSR3 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human SSR3 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunofluorescence (IF)
(Immunofluorescence staining of SSR3 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human SSR3 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.)
Immunofluorescence (IF)
(Immunofluorescence staining of SSR3 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human SSR3 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.)
Immunoprecipitation (IP)
(SSR3 was immunoprecipitated using: Lane A:0.5 mg A431 Whole Cell Lysate 4 uL anti-SSR3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-SSR3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa Observed band size :21 kDa)
Immunoprecipitation (IP)
(SSR3 was immunoprecipitated using: Lane A:0.5 mg A431 Whole Cell Lysate 4 uL anti-SSR3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-SSR3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa Observed band size :21 kDa)
Related Product Information for anti-SSR3 antibody
SSR3 (Signal Sequence Receptor Subunit 3) is a Protein Coding gene. The SSR3 gene, located on 3q25.31, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, and frog. This gene encodes the gamma subunit, which is predicted to span the membrane four times. SSR subunit gamma (SSR3), an SSR family member, is heavily involved in cell growth and differentiation and is closely associated with many tumor types. SSR3 acts as an oncogene in HCC and can therefore serve as a biomarker for the prognoses of HCC patients. SSR3 is widely expressed in the thyroid, endometrium, and other tissues. Diseases associated with SSR3 include Congenital Disorder Of Glycosylation, Type-In.
References
Ng BG, et al. (2019) Mutations in the translocon-associated protein complex subunit ssr3 cause a novel congenital disorder of glycosylation. J Inherit Metab Dis 42 (5): 993-997.Huang S, et al. (2018) Overexpression of signal sequence receptor gamma predicts poor survival in patients with hepatocellular carcinoma. Hum Pathol 81 47-54.Chen HZ, et al. (2013) The orphan nuclear receptor tr3/nur77 regulates er stress and induces apoptosis via interaction with trapgamma. Int J Biochem Cell Biol 45 (8): 1600-1609.
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Product Notes
The SSR3 (Catalog #AAA8108746) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SSR3 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SSR3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC-P: 1:50-1:200 ICC/IF: 1:100-1:500 IP: 0.5-2uL/mg of lysate. Researchers should empirically determine the suitability of the SSR3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SSR3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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